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目的：研究塞来昔布联合X 线在体外环境下对人胆管癌细胞株QBC939 凋亡的影响并对其机制进行初步探讨。方法： CCK-8 检测出不同浓度的塞来昔布及不同剂量的X 线对QBC939 细胞株的增值抑制率，确定塞来昔布组的IC50 及X 线组的 IC50。将QBC939 细胞株分为5 组：对照组（control）、X 线组（R）、塞来昔布组（C）、塞来昔布再加X 线组（C+R）,X 线再加塞来昔布组（R+C）。用流式细胞仪检测各组的凋亡率，western blot 检测凋亡相关基因survivin 蛋白的表达。结果：联合使用塞来昔布和放疗组的凋亡率有明显的增加，从8.268%，11.233%到15.733%，22.133% (P＜0.05)。western 结果显示联合组survivin 蛋白的表达也明显低于对照组(P＜0.05)。先用塞来昔布再加放疗的效果要优于先用放疗后用塞来昔布的效果。结论：塞来昔布联合X 线对 QBC939 细胞株有凋亡增敏作用，作用可能机制之一是通过降低或下调凋亡相关基因survivin 蛋白的表达。

英文摘要:

Objective: To investigated the effect of celecoxib in combination with x-ray on the apoptosis of human cholangiocarcinoma cell line QBC939 in vitro. Methods: The QBC939 cells were cultured in RIMP1640 supplemented with 10% FBS and treated with celecoxib in combination with x-ray. Cell Counting Kit-8 (CCK-8) assay was used to investigate the inhibition ratio and the IC50. Cells were divided into five groups: control group, x-ray group, celecoxib group, x-ray after the celecoxib group and x-ray before celecoxib group. Apoptosis of each group were determined by flow cytometry. Expression of survivin was determined by western blot. Results: The apoptosis ratio of the combination treatment groups enhanced apparently. The efficacy of the x-ray after celecoxib group was more significant than the x-ray before celecoxib group (P＜0.05). Western blot showed that survivin expression was down-regulated obviously after combination treatment. Conclusion: Celecoxib enhanced x-ray's effect of apoptosis-inducing. Celecoxib had the radiosensitivity-enchancing effect on the cholangiocarcinoma QBC939 cells in vitro, one of the possible mechanisms may be that it reduced the expression or accelerating the degradation of survivin.

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