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目的：研究骨桥蛋白OPN 在胰腺癌中的表达水平及其与临床病理特征的关系。方法：采用RT-PCR 和免疫组织化学方法分别检测50 例胰腺癌手术切除标本和20 例癌旁正常胰腺组织中OPNmRNA 及其蛋白水平的表达。结果：胰腺癌组织中OPN mRNA 高表达率为90%（45/50）明显高于其在癌旁正常胰腺组织中的表达15 %（3/20），差异有统计学意义( P<0.01)；OPN 蛋白的阳性率为86.0 %（43/50），明显高于癌旁正常胰腺组织中的表达30%（6/20），差异有统计学意义( P<0.01)；OPN 在胰腺癌组织中表达与其淋巴结转移明显相关（P<0.05）。结论：OPN 在胰腺癌中的过表达对胰腺癌的生长、浸润及转移起重要作用。

英文摘要:

Objective: To study the mRNA and protein expression levels of osteopontin in pancreatic cancer and the relationship with clinic pathological features. Methods: Amount of 50 cases of pancreatic cancer surgical specimens and 20 cases of adjacent normal tissue were studied. The OPN mRNA and protein expression levels were detected by RT-PCR and immunohistochemistry respectively. Results: RT-PCR analysis showed that the OPN mRNA were highly expressed in pancreatic tissues (45/50, 90 % ), which was significantly higher than in adjacent normal tissues (3/20, 15 %) (P<0.01). Immunohistochemistry analysis demonstrated that the OPN protein was high expressed in pancreatic tissues (43/50, 86 %), which was significantly higher than in adjacent normal tissue (6/20, 30 %) (P<0.01). The expression levels of OPN in pancreatic cancer were obviously associated with lymph node metastasis (P<0.05). Conclusion: The OPN was overexpressed in pancreatic cancer, which maybe played important roles in the growth, invasion and metastasis of cancer.

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