| 汪菲高春记△ 黄文荣李晓红李猛.rhG-CSF 动员对供者CD4+ T 细胞免疫表型和功能影响[J].现代生物医学进展英文版,2012,12(4):631-634. |
| rhG-CSF 动员对供者CD4+ T 细胞免疫表型和功能影响 |
| Impact on Immunophenotypes and Functions of CD4+ T Cell DuringMobilization in Donors with rhG-CSF |
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| DOI： |
| 中文关键词: CD4+ T 细胞 rhG-CSF 动员 LFA-1 ICAM-1 |
| 英文关键词: CD4+ T lymphocyte rhG-CSF Mobilization LFA-1 ICAM-1 |
| 基金项目:国家自然科学基金项目（30570776） |
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| 中文摘要: |
| 目的：研究重组人粒细胞集落刺激因子（rhG-CSF）动员对供者CD4+ T 细胞表面分子淋巴细胞功能相关抗原-1（LFA-1）、细
胞间黏附分子-1（ICAM-1）、L- 选择素（LAM-1）和人整合素-4（VLA-4）的表达及其介导的CD4+ T 细胞功能的影响，探讨外周血
干细胞移植过程中CD4+ T 细胞免疫耐受机制。方法：使用三色荧光标记检测动员前及动员后第5 天供者外周血LFA-1、
ICAM-1、LAM-1 和VLA-4 的表达率，ELISA 方法检测动员前后CD4+ T 细胞分泌IFN-γ 和IL-4 能力，免疫磁性分选法分离纯化
CD4+ T 细胞，检测动员前后CD4+ T 细胞对基质细胞衍生因子-1α（SDF-1α）的迁移能力和对ICAM-1 的黏附能力。结果：动员前
后CD4+ T 细胞LFA-1（CD11a）和VLA-4（CD49d）表达率差异无统计学意义（P>0. 01），动员前后CD4+ T 细胞LAM-1（CD62L）和
ICAM-1（CD54）的表达率差异均有统计学意义，动员前显著高于动员后（P<0.01）；动员前后CD4+ T 淋巴细胞向SDF-1α 的迁移率
差异无统计学意义（P>0. 01）；动员后CD4+ T 细胞对ICAM-1 的黏附率降低（P<0.01）；动员后IL-4 和IFN-γ 两个细胞因子在外周
血血清的浓度均降低（P<0.01）。结论：rhG-CSF 动员不影响CD4+ T 细胞LFA-1 和VLA-4 表达及CD4+ T 细胞迁移，但影响CD4+
T 细胞ICAM-1 和LAM-1 表达以及CD4+ T 细胞通过LFA-1 对ICAM-1 的黏附能力影响，并可能影响CD4+ T 细胞分泌细胞因子
IL-4 及IFN-γ 的功能。 |
| 英文摘要: |
| Objective: To investigate the mechanism of CD4+ T cells immunotolerance during allogeneic peripheral blood stem
cell transplantation by studying the impact of recombinant human granulocyte colony stimulating factor (rhG-CSF) on expression of
lymphocyte function antigen-1 (LFA-1), intercellular adhesion molecule (ICAM-1), L-Selectin (LAM-1) and very late antigen 4α
(VLA-4) on CD4+ T cells, and functions of CD4+ T cells through these molecules during mobilization. Methods: The expression of
LFA-1, ICAM-1, LAM-1 and VLA-4 of CD4+ T cells were determined by FACS before rhG-CSF mobilization and the fifth day of
mobilization. The expression of IFN-γ and IL-4 by CD4+ T cells were examined with ELISA. At the same time, the migration and
adhesion activity of CD4+T cells to SDF-1αand ICAM-1 were detected. Results: The expression of LFA-1 on CD4+ T lymphocytes were
both 100%. The expression of VLA-4 didn't change markedly before and the fifth day of mobilization (P>0. 01). The expression of
LAM-1 (CD62L) and ICAM-1 (CD54) on CD4+ T lymphocytes decreased during mobilization (P<0.01). SDF-1α induced CD4+ T
lymphocytes migration for 4 hours didn't change markedly during mobilization (P>0.10). The adhesion activity of CD4+T lymphocytes to
ICAM-1 decreased markedly during mobilization (P>0.10). Conclusion: The concentration of IL-4 and IFN-γ in peripheral blood serum
decreased markedly during mobilization (P<0.01). In conclusions, rhG-CSF does not have effect on the expression of LFA-1 and VLA-4
on CD4+ T cell, and the migration activity of CD4+ T cell during mobilization, but can affect the expression of ICAM-1and LAM-1 on
CD4+ T cell, and the adhesion activity of CD4+ T cell to ICAM-1 through LFA-1. |
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