| 李巨仕邓东湘刘国华△.塞来西布及5- 氟尿嘧啶对人胆管癌QBC939 细胞生长抑制
及诱导凋亡的影响[J].现代生物医学进展英文版,2012,12(3):456-459. |
| 塞来西布及5- 氟尿嘧啶对人胆管癌QBC939 细胞生长抑制
及诱导凋亡的影响 |
| Effect of Celecoxib and 5-FU on Proliferation and Apoptosisof the Human Cholangiocarcinoma QBC-939 Cell Line |
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| DOI： |
| 中文关键词: 塞来西布 5- 氟尿嘧啶 胆管癌 细胞凋亡 细胞周期 |
| 英文关键词: Celecoxib 5-FU Cholangiocarcinoma Apoptosis Cell cycle |
| 基金项目: |
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| 中文摘要: |
| 目的：研究塞来西布(Celecoxib)和5- 氟尿嘧啶（5-FU）对人胆管癌QBC939 细胞生长抑制和凋亡的影响。方法：体外培养人
胆管癌QBC939 细胞，噻唑兰比色实验(MTT)观察Celecoxib 和5-FU 对胆管癌QBC939 细胞生长抑制作用；流式细胞术检测细胞
生长周期和凋亡率改变。结果：不同浓度的Celecoxib 和5-FU 可抑制胆管癌QBC939 细胞的生长，细胞生长抑制率呈时间- 浓度
依赖性(P<0.01)；实验组QBC939 细胞凋亡率随药物浓度的升高逐渐增高(P<0.01)，S 期细胞逐渐减少(P<0.05)，G1 期细胞逐渐增
加(P<0.05)，G2 期细胞无明显变化。结论：Celecoxib 和5-FU 可抑制人胆管癌QBC939 细胞的增殖，诱导其凋亡；联合用药效果优
于Celecoxib 和5-FU 单药效果。 |
| 英文摘要: |
| Objective: To investigate the effect of Celecoxib and 5-FU on proliferation and apoptosis in human cholangiocarcinoma
QBC939 cells line in vitro. Methods: QBC939 cells line were treated with different concentration of Celecoxib or 5-FU, then the
inhibition rate of cell growth were detected by MTT and apoptosis index and cell cycle arrest detected by flowcytometer. Results:
Different concentration of Celecoxib and 5-FU can inhibit proliferation of QBC939 cells line.The inhibitation effect depends on time and
concentration (P<0.01);The apoptosis index of QBC939 gradually increase with the increase of drug concentration (P<0.01)，S stage cell
decreases gradually while G1 stage cell increases (P<0.05), G2 stage cell ratio has no obvious change. Conclusions : Celecoxib and 5-FU
inhibits proliferation of QBC939 cells line and induce apoptosis in vitro. Combination Celecoxib with 5-FU therapy is better than alone. |
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