| 殷小成肖正香彭艳辉赵谦阳声芝刘群策.DADS 上调K562 细胞p21WAF1 表达对细胞生长阻抑和凋亡
的实验研究[J].现代生物医学进展英文版,2012,12(3):439-441. |
| DADS 上调K562 细胞p21WAF1 表达对细胞生长阻抑和凋亡
的实验研究 |
| Effect of Diallyl Disulfide on the Proliferation, Cell Cycle, Apoptosisof Human Leukemia Cell Line K562 |
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| DOI： |
| 中文关键词: DADS K562 细胞 细胞凋亡 基因，p21WAF1 |
| 英文关键词: DADS K562 cells Apoptosis Gen p21WAF1 |
| 基金项目:湖南省高校科研经费项目（09C840） |
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| 中文摘要: |
| 目的：探讨二烯丙基二硫（DADS）对体外培养的人白血病细胞系K562 细胞生长阻抑和凋亡作用及机制。方法：采用MTT
分析法检测细胞活性、流式细胞术分析细胞周期及凋亡率、免疫组化检测p21 WAF1 基因表达。结果：1）. DADS 在10mg / L～80 mg
/ L 范围内，对K562 细胞的抑制作用呈剂量- 时间依赖效应；2）. 不同浓度DADS 作用于K562 细胞24h 后，细胞周期发生了变
化：DADS 可以将K562 细胞阻滞于G2 / M 期；3）. DADS 浓度在10mg / L～80mg / L 时作用K562 细胞24h 后，凋亡率逐渐升高，
有显著的统计学意义（P<0.05 或P<0.01）；4）. 用浓度分别为0mg / L，20mg / L，40mg / L，80mg / L 处理K562 细胞24h 后，p21 WAF1 蛋
白表达上调，有统计学意义（P<0.05 或P<0.01），溶媒组和阴性对照组无差别（P>0.05）。结论：DADS 有抑制K562 细胞增殖和促
进K562 细胞凋亡的作用。其作用的可能机制与上调细胞周期蛋白依赖性激酶抑制剂p21WAF1 表达，从而诱使k562 细胞阻滞于
G2/M 期有关。 |
| 英文摘要: |
| Objective: To explore the mechanism of cell proliferation-inhabit and apotosis of human leukemia cell line K562
treated with diallyl disulfide (DADS). Methods: MTT assay，flow cytometry and immunohistochemistry were employed to examine
DADS-induced apoptosis and growth inhibition in human Leukemia cell Line K562. Rusults：1.DADS inhibited proliferation of K562
cells from 10～80mg.L-DADS in a dose-and time-dependent manner ; 2 .K562 cells treated with DADS after the treatment for 24 hours
significantly increased in the percentage of cells in the G2/M phase; 3.From 10～80mg.L-DADS induced apotosis of K562 cells after the
treatment for 24 hours, the difference is significant;4. DADS could up-regulate the level of protein of p21WAF1 in K562 cells. Conclusions：
DADS has apparent effects of proliferation-inhabit and apoptosis on K562 cells in vitro, its anti-leukemia mechanisms may be related to
up-regulate the gene of of p21WAF1 in K562 cells. |
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