| 胡艳妹1,2 夏彪1 丁颖1 王宁1 殷丹丹1 德伟1△.c-met 在大鼠胰岛β细胞INS-1 胰岛素分泌中的作用[J].现代生物医学进展英文版,2012,12(3):435-438. |
| c-met 在大鼠胰岛β细胞INS-1 胰岛素分泌中的作用 |
| The Role of c-met Expression in INS-1 Rat Pancreatic Beta CellsIn Insulin Secretion |
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| DOI： |
| 中文关键词: c-met 胰岛β 细胞 GSIS GLUT2 |
| 英文关键词: c-met β-cell GSIS GLUT2 |
| 基金项目: |
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| 中文摘要: |
| 目的：从c-met 对胰岛β 细胞增殖，细胞周期、糖耐受和对GLUT2 的表达影响三个方面探讨c-met 在胰岛β 细胞功能的
影响及相关机制。方法：在大鼠胰岛β 细胞系INS-1 中运用RNA 干扰技术(RNAi)抑制HGF 的特异性受体c-met 蛋白的表达，
检测其在正常的生理状况下对成熟的胰岛β 细胞增殖以及功能维持的作用。结果：c-met 蛋白对成熟的胰岛β细胞的增殖与周
期并没有显著影响，但对于β 细胞的功能维持具有重要意义。结论：通过调节GLUT2 蛋白来维持β细胞的胰岛素分泌功能，有
助于进一步阐明HGF/c-met 通路在胰岛β 细胞功能损伤的分子机制，从而为糖尿病的预防和治疗提供新的理论依据。 |
| 英文摘要: |
| Objective: To investigate the effect and mechanism of c-met on the function of pancreatic beta cells in three aspects:
β-cell proliferation, cell cycle, glucose tolerance and the impact of GLUT2. Methods: Use RNA interference (RNAi) to inhibit the
expression of c-met protein, HGF-specific receptor, in rat pancreatic β cell line INS-1, and detect the effect on the mature β-cell
proliferation and function maintenance in normal physiological conditions. Results: There is no significant impact of c-met protein on
mature pancreatic β-cell proliferation and cell cycle, but it shows great effect on maintaining the function of β-cell. Conclusion: By
regulating GLUT2 protein to maintain β-cell insulin secretion function,contributing to further clarify HGF/c-met pathway in the
molecular mechanism of pancreatic β-cell dysfunction, and providing a new theoretical basis on the prevention and treatment of
diabetes. |
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