| 苏华科1 宋耀明2△ 郭文昀2 李文军1 刘启亮2.西洛他唑对大鼠颈总动脉球囊损伤后内膜增生及氧化应激的影响[J].现代生物医学进展英文版,2012,12(2):247-249. |
| 西洛他唑对大鼠颈总动脉球囊损伤后内膜增生及氧化应激的影响 |
| Effects of Cilostazol on Oxidative Stress and Neointimal Hyperplasiaafter Balloon Injury in Rat Model |
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| DOI： |
| 中文关键词: 氧化应激 西洛他唑 球囊损伤 再狭窄 |
| 英文关键词: Oxidative stress Ccilostazol Balloon injury Restenosis |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨西洛他唑对大鼠颈总动脉球囊损伤后内膜增生的抑制作用和血管壁氧化应激的影响。方法：SD 大鼠24 只，随机
分组：假手术组、损伤组及西洛他唑治疗组。采用球囊损伤大鼠左侧颈总动脉，于术后2 周处死大鼠，取损伤血管标本，进行HE 染
色、免疫组化染色及原位DHE 染色，检测内膜增生、平滑肌细胞增殖及血管壁局部ROS 水平。结果：球囊损伤2 周后，血管壁内膜
显著增生，西洛他唑治疗后内膜增生显著抑制，两组相比P<0.05。PCNA 免疫组化染色：假手术组未见PCNA 阳性细胞，损伤组
PCNA 阳性细胞面积百分比明显高于西洛他唑组，主要分布于新生内膜和内弹力膜区域（P<0.05）。原位DHE 染色：球囊损伤后
局部ROS 水平显著升高，较假手术组差异显著P<0.05，西洛他唑干预后局部ROS 水平显著降低（P<0.05）。结论：新型抗血小板制
剂西洛他唑可显著抑制大鼠颈总动脉球囊损伤后内膜增生及局部氧化应激，抑制局部氧化应激可能是西洛他唑抑制内膜增生的
机制之一。 |
| 英文摘要: |
| Objective: To investigate the effect of cilostazol on oxidative stress and neointimal hyperplasia after balloon injury in
rat model. Methods: Twenty-four male SPF SD rats were randomly divided into three groups: sham-operation group, injury group and
cilostazol group(n=8). Except shan-operation group, all Left common carotid artery of rats were injuried by a balloon catheter. 14days later,
the injured carotids were sliced, HE and immunohistochemistry stained. The level of reactive oxygen species (ROS) was examined by
Dihydroethidium (DHE). Ratios of intima/media area, PCNA positive indexes and the level of ROS were compared. Results: The ratios
of intima/media area, PCNA positive indexes and the level of ROS in injury group were significantly higher than that in sham-operation
group (P<0.05). Cilostazol can significantly decrease the ratios of intima/media area, PCNA positive indexes and the level of ROS(P<0.
05). Conclusion: Cilostazol reduces neointimal hyperplasia by inhibition superoxode production after balloon common arterial injury in a
rat model. |
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