| 张志明孙苏平.重组人内皮抑素对食管癌细胞生长抑制作用的体外研究[J].现代生物医学进展英文版,2012,12(2):235-238. |
| 重组人内皮抑素对食管癌细胞生长抑制作用的体外研究 |
| Growth Inhibition of Esophageal Cancer Cell in vitro by rhES |
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| DOI： |
| 中文关键词: 重组人内皮抑素 食管癌细胞 生长抑制 |
| 英文关键词: rhES Esophageal squamous carcinoma Growth inhibition |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨重组人内皮抑素(rhES)对食管鳞癌系KYSE-150 及TE1 细胞生长的影响。方法：应用四甲基偶氮唑盐( MTT ) 法，
以大鼠成纤维细胞L929 为对照细胞，检测rhES 不同浓度（0、12.5、25、50、100、200、400、600 μg/ml）和作用时间（24h、48h）对食管
鳞癌系KYSE-150、TE1 和L929 细胞的生长抑制作用。结果：大鼠L929 细胞经rhES 处理后，吸光度A 值轻微降低，差异不具有
统计学意义(P＞0.05)，食管鳞癌系KYSE-150、TE1 经rhES 处理后，吸光度A 值明星降低，差异具有统计学意义(P＜0.05)。结论：
rhES 明显抑制食管鳞癌系KYSE-150 及TE1 细胞增殖, 且呈时间- 剂量依赖性。 |
| 英文摘要: |
| Objective: To investigate the growth inhibition of Recombinant Human Endostatin (rhES) on human esophageal squamous
carcinoma cell line KYSE-150 and TE1. Methods: The effect of rhES on the proliferation of human esophageal squamous carcinoma
cell line KYSE-150, TE1 cell and of normal cell line (L929) was studied by3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide(
MTT) assay, treating with various concentrations (0, 12.5, 25, 50, 100, 200, 400, 600 μg/ml) and at different time points (24h, 48h).
Results: After treating with various concentrations of rhES and at different time points, the absorbance of human esophageal squamous
carcinoma cell line KYSE-150, TE1 was significantly decreased (P＜0.05) while L929 was not(P＞0.05). Conclusions: rhES can inhibits
the growth of human esophageal squamous carcinoma cell line KYSE-150, TE1 with time-dose dependent style. |
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