| 曹颖金哲△ 于妍妍徐翠.成人宫颈上皮细胞的原代培养及鉴定[J].现代生物医学进展英文版,2012,12(2):204-206. |
| 成人宫颈上皮细胞的原代培养及鉴定 |
| Primary Culture and Identification of Adult Cervical Epithelial Cells |
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| DOI： |
| 中文关键词: 宫颈上皮细胞 原代培养 无血清培养基 |
| 英文关键词: Cervical epithelial cell Primary culture Serum-free medium |
| 基金项目:国家自然科学基金面上项目(30873278) |
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| 中文摘要: |
| 探索采用无血清培养基原代培养成人宫颈上皮细胞的方法。方法：以成人的宫颈上皮组织为研究对象，采用胰蛋白酶
-EDTA 消化法获得宫颈上皮细胞悬液，于上皮细胞专用无血清培养基中培养，采用免疫细胞化学法测定细胞中角蛋白及波形蛋
白的表达，对细胞纯度进行鉴定。结果：原代培养10-15 天细胞融合达60%，传代至4-6 代，细胞出现生长衰退。早期细胞生长状态
良好，细胞纯度在90%以上。结论：采用酶消化法及K-SFM 无血清培养基培养可获得纯度高的成人宫颈上皮细胞。 |
| 英文摘要: |
| Objective: To investigate the primary culture method of adult cervical epithelial cells using serum-free keratinocyte
medium. Methods: Adult cervical epithelial tissue pieces was obtained and digested in typsin-EDTA to dissociate the cells and the single
cell suspension was got. The cells were cultured in serum-free keratinocyte medium. The expression of keratin and vimentin was assayed
by immunohistochemical staining method respectively to identify the cell purity. Results: The primary cells reach about 60% until 10 to
15 days following isolation and setup, the cells usually senesced at passage 4 to 6. The early passages cells grew well ,cell purity was at
least 90%. Conclusion: High purity rate adult cervical epithelial cells can be obtained by this kind of primary culture method. |
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