| 栗莉张磊候祥红曹瑞季爱玲王枫.TLR4 对人骨骼肌细胞炎症因子表达及胰岛素抵抗的影响[J].现代生物医学进展英文版,2012,12(2):201-203. |
| TLR4 对人骨骼肌细胞炎症因子表达及胰岛素抵抗的影响 |
| Effects of TLR4 on the Expression of Inflammatory Factors and InsulinResistance in Human Skeletal Muscle Cells |
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| DOI： |
| 中文关键词: TLR4 人骨骼肌细胞(HSKMC 3500) 炎症因子 胰岛素抵抗 |
| 英文关键词: TLR4 Human skeletal muscle cell Inflammatory factors Insulin resistance |
| 基金项目:国家自然科学基金资金项目（81072303） |
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| 中文摘要: |
| 目的：研究TLR4 对脂多糖（LPS）及Polymymin B (PMB)作用下的人骨骼肌细胞的炎症因子表达的影响及其在细胞胰岛素
抵抗中的作用。方法：通过脂多糖（LPS）及Polymymin B (PMB)干预骨骼肌细胞24h，再用胰岛素刺激1h 后，Real-time PCR 检测检
测骨骼肌细胞TLR4、MyD88、TNF-α mRNA 的表达；Western blot 检测TLR4，Myd88 和CRP 的表达；葡萄糖氧化酶法（GOD-POD
法）检测细胞培养液中葡萄糖浓度。结果：TLR4 高表达可以使炎症因子的表达增高，细胞培养液中的葡萄糖浓度增高；TLR4 低表
达可使炎症因子的表达降低，细胞培养液中的葡萄糖浓度没有明显变化。结论：TLR4 调控了炎症因子的表达，继而可以引起胰岛
素敏感性的改变，影响了胰岛素抵抗的发生。 |
| 英文摘要: |
| Objective: To investigate the effects of TLR4 on the expression of inflammatory factors and insulin resistance in human
skeletal muscle cells. Methods: Cultured human skeletal muscle cells were treated by LPS and PMB for 24h, then the cells were incubated
in insulin for 1h. The expression of TLR4, Myd88, TNF-α was examined by Real-time PCR and the expression of TLR4, Myd88,
CRP were detected byWestern Blot; GOD-POD method was used to assess the concentration of glucose. Results: The activation of TLR4
increased the expression of inflammatory factors and the concentration of glucose; The blocking of TLR4 inhibit the expression of inflammatory
factors, the concentration of glucose had no significantly changes. Conclusion: TLR4 regulates the expression of inflammatory
factors, then could alter the sensibility of insulin. |
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