| 曾丽萍冯吉龙艳△ 孟金陵.油菜硫甙性状QTL 区间的加密和整合[J].现代生物医学进展英文版,2012,12(1):52-55. |
| 油菜硫甙性状QTL 区间的加密和整合 |
| Fine Mapping and Integration of Glucosinolate QTL in Brassica Napus |
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| DOI： |
| 中文关键词: 油菜 硫甙 QTL |
| 英文关键词: Brassica napus Glucosinolate QTL |
| 基金项目: |
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| 中文摘要: |
| 目的：加密油菜控制硫甙性状QTL 区间，并进行QTL 整合预测候选基因。方法：利用生物信息学方法根据已知测序的白菜
BAC 序列信息设计引物，在油菜TN DH 群体中进行多态性扩增和定位，并根据加密后构建的遗传连锁图重新检测QTL，进行
QTL 整合。结果：将根据白菜BAC 设计的3 对多态性标记成功定位到油菜控制硫甙性状QTL 区间，进行QTL 整合后将QTL 置
信区间进一步缩小，并判定了初步的候选基因。结论：充分利用白菜已测序的BAC 或者基因组信息，将能加快油菜基础研究的步
伐。 |
| 英文摘要: |
| Objective: Adding markers to the confidence interval of glucosinolate content QTL in Brassica napus, and then to do
QTL integration and candidate gene prediction. Methods: Designed primers according to BAC sequence of Brassica rapa, and then detected
polymorphism between the two parents of TN DH population of B.napus. With the polymorphic markers genetic mapping will be done in
TN DH population. Finally, QTL mapping and integregation will be done based on the new linkage map. Results: Three polymorphic
markers were mapped to the confidence interval of seed glucosinolate content QTL, after QTL mapping and integregation were done, the
confidence interval was narrow down and two candidate genes were predicted in the region. Conclusion: If taking full advantage of the
sequenced BAC and whole genomic information, the basic research of B.napus will be speeded up. |
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