| 宋毕清时开网△ 杨士勇.米非司酮对胰腺癌细胞耐药株Patu8988/FU 的逆转作用[J].现代生物医学进展英文版,2012,12(1):20-22. |
| 米非司酮对胰腺癌细胞耐药株Patu8988/FU 的逆转作用 |
| Study of Mifepristone on Reversing Multidrug Resistance of HumanPancreatic Cancer Cell Line Patu8988/FU |
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| DOI： |
| 中文关键词: 米非司酮 胰腺癌 耐药逆转 |
| 英文关键词: Mifepristone Pancreatic cancer Multidrug resistence |
| 基金项目:江苏省自然科学基金（H200945） |
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| 中文摘要: |
| 目的：研究米非司酮（mifepristone, MIF）对胰腺癌多药耐药细胞株Patu8988/FU 耐药性的逆转作用。方法：采用四甲基偶氮
唑盐（MTT）比色法检测MIF 对胰腺癌细胞Patu8988 及胰腺癌耐药细胞株Patu8988/FU 增殖的影响，选择对两种细胞生长抑制
率≤5 %的浓度为其非细胞毒性剂量；观察非毒性剂量的MIF 作用72 h 后Patu8988/FU 细胞对5-FU 耐药性的逆转效果。结果：①
MIF 浓度≤10 μmol.L-1 对Patu8988 及Patu8988/FU 于细胞的增殖均无抑制作用（P＞0.05）；20 μmol.L-1 和40μmol.L-1 MIF 对
Patu8988 及Patu8988/FU 于细胞的增殖均有抑制作用（P＜0.05），MIF 对Patu8988 及Patu8988/FU 细胞增殖具有剂量依赖性；②
加入梯度浓度5-FU 后Patu8988 细胞和Patu8988/FU 细胞的IC50 分别为（2.394±0.011）μg.ml-1、（49.87±4.026）μg.ml-1 （P＜
0.01），耐药倍数为20.83；Patu8988 及Patu8988/FU 细胞加入5-FU 后分别与2.5 μmol.L-1、5μmol.L-1、10 μmol.L-1 MIF 共同孵育72
h 后，Patu8988/FU 细胞对5-FU 耐药性的逆转倍数分别为1.42、1.71、3.10；Patu8988/FU 对5-FU 的敏感性明显增加（P<0.05），而
Patu8988 细胞对5-FU 的敏感性并未改变（P＞0.05）。结果还显示MIF 作用后Patu8988/FU 对5-FU 的敏感性变化具有剂量依赖
性。结论：MIF 对胰腺癌多药耐药细胞株Patu8988/FU 耐药性的有逆转作用。 |
| 英文摘要: |
| Objective: To study whether mifepristone could reverse multidrug resistance of Patu8988/FU cells. Methods: MTT was
used to study the proliferation and sensitivity of Patu8988 and Patu8988/FU cell lines, to chose non-cytotoxic concentrations by making
the inhibition rate of these two cell lines less than 5%, to observe the resersal of drug resistance of 5-FU after 72 hours treatment with
miferpristone of non-cytotoxic concentrations. Results: ① Miferprestone with concentration of no more than 10 μmol.L-1 did not affect
the proliferation of Patu8988 and Patu8988/FU cell lines (P>0.05), but 20 μmol.L-1,40 μmol.L-1 miferprestone did (P<0.05). Miferprestone
could dose-dependently inhibit the prolification of Patu8988 and Patu8988/FU cells. ② IC50 of 5-FU to Patu8988 and
Patu8988/FU cells were (2.394±0.011) μg.ml-1 and (49.87±4.026) μg.ml-1, respectively,(P<0.01), the resistance multiples of 5-FU were
20.83 in Patu8988/FU cell lines. After 2.5 μmol.L-1, 5 μmol.L-1, 10 μmol.L-1 miferprestone added, the reversal multiples of drug resistance
were 1.42, 1.71, 3.10 ,respectively. Miferprestone increased the sensitivety of Patu8988/FU to 5-FU (P<0.05), while did not change
that of Patu8988(P>0.05). Conclusion: Miferprestone can reverse the drug resistance on Patu8988/FU cell lines. |
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