| 杨荣秉梁伟连觅徐兰初明朱蕴兰余培峰徐碧荷王月丹.肿瘤坏死因子α和甲基化转移酶抑制siRNA 对Raji 细胞中癌- 睾丸抗
原表达的影响[J].现代生物医学进展英文版,2012,12(1):-1. |
| 肿瘤坏死因子α和甲基化转移酶抑制siRNA 对Raji 细胞中癌- 睾丸抗
原表达的影响 |
| Effect of TNF-α and RNAi on the Expression File of Cancer-TestisAntigens in Raji Cell Line |
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| DOI： |
| 中文关键词: TNF-α siRNA 癌- 睾丸抗原 DNA 甲基化转移酶 |
| 英文关键词: TNF-α SiRNA Cancer-Testis Antigens（CTA） DNMT |
| 基金项目:国家自然科学基金资助项目（30771995） |
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| 中文摘要: |
| 目的：研究细胞因子TNF-α 和针对DNA 甲基化转移酶的siRNA 对Burkitt 淋巴瘤细胞株Raji 细胞的癌- 睾丸抗原表达情
况的影响。方法：1.加入不同终浓度的细胞因子TNF-α，与Raji 细胞株共培养，在不同的时间点检测癌- 睾丸抗原表达情况的改
变。2.转入针对DNA 甲基化转移酶的siRNA，检测癌- 睾丸抗原表达情况的改变。结果：在TNF-α 作用后，一些细胞组会出现
SSX-1 表达上调，而siRNA 干扰作用后，转染针对DNMT1 和DNMT3b 的细胞组或受5-aza-dC 作用的细胞组的PRAME 发生再
表达。结论：TNF-α 和siRNA 都能诱导某些癌睾丸抗原的表达或使其表达增强。 |
| 英文摘要: |
| Objective: To investigate the effect of TNF- α and RNA interference of DNMTs on the expression file of Cancer-
Testis Antigens in Burkitt lymphoma cell line Raji. Methods: The expression file of Cancer-Testis antigens mRNA in Raji cell treated
with TNF-α and transfected with siRNA was detected by RT-PCR respectively. Results: After treated by TNF-α, the expression of SSX-1
and NY-ESO-1 were upegulated in some groups, while PRAME was re-expressed in groups transfected with siRNA towards DNMT1 and
DNMT3b or 5-aza-dC. Conclusion: TNF-α and siRNA have effect on the expression level of Cancer-Testis Antigens in Raji cell line. |
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