毕书婷1 陈石2 葛海波2 李田2 姚欣1 黄茂1.肿瘤坏死因子α-863C/A基因多态性与慢性阻塞性肺疾病的
相关性研究[J].现代生物医学进展英文版,2011,11(21):4098-4102. |
肿瘤坏死因子α-863C/A基因多态性与慢性阻塞性肺疾病的
相关性研究 |
Relationship between Gene Polymorphisms of Tumour NecrosisFactor-α-863C/A and Chronic Obstructive Pulmonary Disease |
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DOI: |
中文关键词: 肿瘤坏死因子α基因 基因多态性 慢性阻塞性肺疾病 |
英文关键词: Tumor necrosis factor-α Gene polymorphisms Chronic obstructive pulmonary disease |
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中文摘要: |
目的:探讨肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)-863C/A 基因多态性与慢性阻塞性肺疾病(chronic obstructive
pulmonary disease, COPD)间的关系。方法:采用多重聚合酶链- 高温连接酶检测反应(PCR-LDR)方法研究56 例吸烟COPD 患
者、64例吸烟正常对照组的TNF-α-863位点基因型及A等位基因的分布情况。结果:(1)吸烟COPD 组在-863 位点上的CA 基
因型及A等位基因频率均低于吸烟非COPD组(P<0.05)。(2)重度、极重度COPD组在该位点的CA基因型频率和A等位基因频
率均低于轻中度COPD组(P<0.05)。在COPD组中携带A 等位基因的患者有着更高的第1 秒呼气量/ 用力肺活量(FEV1/FVC)
和体重指数(BMI)(P<0.05)。(3)吸烟COPD 组携带CA 基因型的患者肺源性心脏病的发病率高于携带CC 基因型的患者(P<0.
05)。(4)吸烟COPD组的BMI[(22.0±3.1)kg/m2]较吸烟非COPD 组[(24.5±3.7)kg/m2]低,重度、极重度COPD 组BMI[(21.2±3.0)
kg/m2]较轻中度COPD 组[(23.3±3.3)kg/m2]低,差异均有统计学意义(P<0.05)。结论:TNF-α-863 位点A 等位基因的发生对
COPD患者是一个保护性因素。低BMI与COPD关系密切,BMI可能成为预测COPD患者病情严重程度的一个重要指标。 |
英文摘要: |
Objective: To study the relationship between tumor necrosis factor-α-863C/A gene polymorphisms and chronic obstructive
pulmonary disease (COPD). Methods: Polymerase chain reaction-ligase detection reaction-based sequence analysis was applied
to determine the frequencies of genotypes and allele of TNF-α-863 in 56 cases of patients with smoking-related COPD and a control
group of 64 smokers. Results: The frequencies of TNF-α-863 CA and A allele in COPD patients were lower than the control group (P<0.
05), and the severe subgroups were lower than non-severe subgroups (P<0.05). The CA genotype was associated with a better FEV1/FVC
ratio, and higher BMI (P<0.05). The prevalence of chronic pulmonary heart disease in the CA genotypes was higher than the CC genotypes
in COPD group (P<0.05). Compared with non-COPD subjects, BMI was lower in COPD patients[(22.0±3.1) vs (24.5±3.7)kg/m2,
P<0.05]; BMI was also lower in the severe subgroups[(21.2±3.0) vs (23.3±3.3)kg/m2, P<0.05]. Conclusions: The TNF-α-863 A allele
may be a protective factor for COPD. Lower BMI was strongly associated with COPD, possibly as a potential predictor for COPD severity. |
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