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目的：探讨125I-UdR 提高内皮抑素基因对大鼠种植癌模型的抑制效应。方法：制作肿瘤株Walker-256 细胞大鼠皮下种植癌的动物模型并分为1-4 组（对照组、125I-UdR 组、Endostatin 组和Endostatin+125I-UdR 组），每组20 只，通过实体瘤内注射，分别给与相同体积生理盐水125I-UdR、内皮抑素基因及125I-UdR 和内皮抑素基因混合物，测量肿瘤治疗前体积（V0）和治疗后不同时间体积（Vt），计算10d、20d 的肿瘤生长率（f= Vt/V0），观察各组肿瘤在光镜下的变化。结果：各组大鼠10d、20d 肿瘤生长率为：（11.03± 1.08、27.35±1.08），（4.02±0.79、7.58±2.98），（3.88±0.26、7.02±2.75），（2.72±1.01、2.94±1.26），2、3、4 组的肿瘤生长率明显小于1 组（P<0.001）；2、3 组之间肿瘤生长率差别不明显（P＞0.05），4 组肿瘤生长率小于2、3 组（P<0.01）。结论：通过实体瘤内注射的方法给与125I-UdR、内皮抑素基因及两者混合物后，能够明显抑制肿瘤的生长，内皮抑素基因和125I-UdR 联合治疗在抑制肿瘤生长方面作用更加显著。

英文摘要:

Objective: To investigate the anti-tumor effect of combinating of endostatin gene therapy with 125I-UdR on rats with implanted tumor. Methods: Immediate Walker-256 cancerous ascetic injection method was used to build up a rat tumor-bearing model, then the rats were randomly divided into groups of 1-4, in which the tumor was treated with saline, 125I-UdR, endostatin gene, endostatin gene plus 125I-UdR, respectively. The morphological changes and tumor growth rate were detected. Results: The tumor growth rate of 10d and 20d in each group was （11.03±1.08, 27.35±1.08）,（4.02±0.79, 7.58±2.98）, （3.88±0.26、7.02±2.75）, （2.72±1.01, 2.94± 1.26）, the rates of group 2-4 were less than that of group 1 (P<0.001). There was no distinguished different between the rates of group 2 and 3 （P＞0.05）. The rate of group 4 was less than those of group 2 and 3 （P<0.01）. Conclusions: The tumor growth was inhibited significantly after the injaction of 125I-UdR, endostatin gene and the composition of 125I-UdR and endostatin gene. However, the effect of the endostatin gene plus 125I-UdR was obviously better than that of the endostatin gene therapy group or the 125I-UdR group for inhibiting tumor growth.

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