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目的：研究小茴香对肝纤维化大鼠的脂质过氧化水平的影响。方法：取Wister大鼠100只，随机取12 只设为正常组，其余采用四氯化碳（CCL4）复合因素法复制肝纤维化大鼠模型，复制成功后随机分为模型对照组、小茴香组和复方丹参组（n=12）；相应药物干预8 周后，镜检肝组织形态学改变；全自动生化分析仪检测外周血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）水平与血清总蛋白（TP）、白蛋白（ALB）含量；生化法检测肝组织羟脯氨酸（HYP）与血清丙二醛（MDA）含量及超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-PX）、过氧化氢酶（CAT）活性。结果：HE 结果显示：模型组肝脏炎症与脂肪变性明显，经小茴香干预后明显改善。生化检测结果显示：与正常组比较，模型组ALT、AST 水平显著升高（P＜0.05），TP、ALB 含量显著降低（P＜0.05），HYP、 MDA 含量显著增加（P＜0.05），而SOD、GSH-PX 和CAT 活性显著降低（P＜0.05）；与模型组比较，小茴香组ALT、AST 水平显著下降（P＜0.05），TP、ALB 水平显著升高（P＜0.05）；HYP、MDA 含量显著降低（P＜0.05），SOD、GSH-PX 和CAT 活性显著提高（P＜0.05）。结论：小茴香的抗肝纤维化作用可能与调节大鼠脂质过氧化水平有关。

英文摘要:

Objective: To observe the effects of foeniculum vulgare mill on liver fibrosis. Methods: 100 Wister rats were randomly divided into 4 groups as control group，model group, foeniculum vulgare mill group, and danshen group. Liver fibrosis models were established by the combination of injecting carbon tetrachloride (CCL4) subcutaneously. Drugs were lavaged for 8 weeks, rats livers tissue were examined with HE staining; the serum level of alanine transaminase (ALT), aspartate aminotransferase(AST), albumin(ALB) and total protern (TP) were determined using corresponding test kits; the hepatic lipid peroxidation indexes, including malondialdehyde (MDA), Superoxide dismutase(SOD),Glutathione peroxidase(GSH-PX),Catalase(CAT) were measured by biochemical methods; Results: Compared with normal group, ALT, AST level and MDA content remarkablely increased, level of TP, ALB and SOD activity decreased (P<0.05). Compared with model group, Foeniculum Vulgare Mill group ALT, AST level and MDA content remarkablely decreased, level of TP, ALB and SOD, GSH-PX, CAT activity remarkablely increased (P<0.05). Conclusions: Foeniculum Vulgare Mill can inhibit the hepatic fibrosis and it's probably mechanism is associated with the effect of Foeniculum Vulgare Mill regulate the lipid peroxidation in the liver.

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