张毅1 陈欣钦2 宋昌彦3.2010 年上海市食源性沙门菌菌型分布和药敏分析
以及快速检测方法的建立[J].现代生物医学进展英文版,2011,11(20):3938-3941. |
2010 年上海市食源性沙门菌菌型分布和药敏分析
以及快速检测方法的建立 |
Foodborne Salmonella Serotype Distribution and Sensitivity Analysis inShanghai in 2010 |
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DOI: |
中文关键词: 沙门菌属 药敏分析 耐药性 侵袭蛋白A |
英文关键词: Saimonella Analysis of drug sensitivity Drug resistance InvA |
基金项目: |
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中文摘要: |
Foodborne Salmonella Serotype Distribution and Sensitivity Analysis in
Shanghai in 2010 |
英文摘要: |
Objective: To provide scientific basis for controlling diseases caused by saimonellas through understanding their
pollution states, distribution of bacterial types and drug resistances of market foods in Shang Hai, and we establishrapid detection method
for Salmonella. Methods: 840 food Salmonella isolation identification and drug resistance analysis from The farmers market and
supermarket, the primers according to the Salmonella typhi invasion protein A(invA)was designed and was PCR to detect the Salmonella.
Results: In all food relevance ratio of saimonellas is 4.29%,the highest is 91% in raw livestock meat.Main serotype Salmonella typhimurium
is salmonella typhimurium, del weakpulse,duplin.The strains is antibiotics sensitive to ceftazidime,but is resistance to ampicillin,midecamycin,
ciprofloxacin, nitrofurantoin,And on this basis through PCR method successfully specificity detection of Salmonella. Conclution:
saimonellas pollution of market foods in Shanghai is predominated covered by raw livestock meat.Current treatment of market
food in foodborne Salmonella foodborne disease should be the first choice of the antibiotic, and PCR rapid detection method also has the
advantages of simple operation, strong specificity, high sensitivity of Salmonella contamination in food can play a quick detection and
monitoring. |
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