| 费哲为欧敬明董谦刘颖斌方国恩.重组人类生长激素对胃癌细胞的体外增殖研究[J].现代生物医学进展英文版,2011,11(20):3849-3852. |
| 重组人类生长激素对胃癌细胞的体外增殖研究 |
| Effects of Recombinant Human Growth Hormone on Growth of HumanGastric Cancer Cell Line in Vitro |
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| DOI： |
| 中文关键词: 重组人类生长激素 胃癌细胞 增殖 |
| 英文关键词: Recombinant human growth hormone (rhGH) Human gastric cancer cell line Growth |
| 基金项目: |
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| 中文摘要: |
| 目的：通过体外实验，研究重组人类生长激素对胃癌细胞的增殖的影响。方法：实验分为空白组，重组人类生长激素组，奥沙
利铂组和重组人类生长激素+ 奥沙利铂组。用不同浓度的重组人类生长激素处理SGC-7901 细胞，采用MTT 法和流式细胞仪检
测人胃癌细胞株的细胞抑制率，细胞周期和DNA 抑制率。结果：体外实验结果表明，重组人类生长激素对SGC-7901 细胞株增殖
没有明显的促进作用，重组人类生长激素组和空白组以及重组人类生长激素+ 奥沙利铂组和奥沙利铂组之间没有统计显著性
(P>0.05)，细胞抑制率和停止生长的细胞在G0-G1 期明显增加（P＜0.01），同时重组人类生长激素+ 奥沙利铂组和空白组以及奥
沙利铂组在S 期，细胞数依次下降，DNA 抑制率依次增加。重组人类生长激素+ 奥沙利铂组与奥沙利铂组相比，细胞抑制率有明
显上升趋势。结论：体外实验表明，重组人类生长激素并不加快人类胃癌细胞的增殖，与抗癌药物一同使用时，有增加治疗功效的
作用。 |
| 英文摘要: |
| Objective: To investigate the effects of recombinant human growth hormone (rhGH) on growth of human gastric
cancer cell line in vitro. Methods: Cells were divided into control group, rhGH group, oxaliplatin (L-OHP) group and rhGH+L-OHP
group. Cell inhibitory rate, cell cycle and DNA inhibitory rate of human gastric cancer line SGC-7901, at different concentrations of
rhGH treatment were detected by cell culture, MTT assay and flow cytometry. Results: There was not distinctly accelerated effect of
rhGH on multiplication of SGC-7901 cell line in vitro. There was no statistical significance between rhGH group and control group, or
between rhGH+L-OHP group and L-OHP group (P>0.05). Cell inhibitory rate and cell arrested in G0-G1 phase increased. Meanwhile,
cells in S phase distinctly decreased and DNA inhibitory rate increased in rhGH+L-OHP group in comparison with L-OHP group （P＜
0.01）. Conclusion: rhGH does not accelerate the multiplication of human gastric cancer cells. It may increase the therapeutic efficacy
when used in combination with anticancer drugs. |
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