| 张大鹏1 张圣洁1 于大淼2 富勇1.Stathmin/Oncoprotein 18(Op18)基因在人骨肉瘤中的表达及其意义[J].现代生物医学进展英文版,2011,11(19):3709-3712. |
| Stathmin/Oncoprotein 18(Op18)基因在人骨肉瘤中的表达及其意义 |
| Expression and Significance of Stathmin/Oncoprotein 18(Op18) Gene inHuman Osteosarcoma |
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| DOI： |
| 中文关键词: 骨肉瘤 stathmin/oncoprotein 18 免疫组化法 Western blot |
| 英文关键词: Osteosarcoma Stathmin/oncoprotein 18 Western blot Immunohistochemical staining |
| 基金项目:黑龙江省教育厅科学技术研究项目（No. 11551168） |
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| 中文摘要: |
| 目的：探讨stathmin/oncoprotein 18 (Op18)基因在人骨肉瘤中的表达规律及意义。方法：分别采用免疫组化法和Western blot
法检测10 例正常骨组织和56 例骨肉瘤中stathmin 蛋白的表达。结果：免疫组化法检测stathmin 蛋白在正常骨组织、低级别骨肉
瘤(G1 级组)及高级别骨肉瘤(G2 级组)中阳性表达率分别为20 %、65 %、100 %。正常骨组织分别与G1 级组、G2 级组比较，均有显
著性差异(P﹤0.05)；G1 级组与G2 级组比较，有显著差异(P<0.05)。Western blot 法检测显示，stathmin 蛋白在正常骨组织、G1 级
组、G2 级组表达相对值分别为（0.34±0.15）、（0.68±0.21）、（0.90±0.17）。正常骨组织分别与G1 级级组、G2 级组比较，差异均有
显著性( P <0.01)，G1 级组与G2 级组比较，差异有显著性(P<0.01)。结论：Stathmin 在骨肉瘤中过表达，与骨肉瘤的发生及发展密
切相关。 |
| 英文摘要: |
| Objective: To investigate the expression of stathmin/ oncoprotein 18 (Op18) in human osteosarcoma and its relation to
biological behaviors of osteosarcoma. Methods: The stathmin protein expressions of 56 cases with human osteosarcoma and 10 cases of
normal bone tissues were examined by immunohistochemical staining and Western blot methods. Results: The positive rate of stathmin in
normal human bone tissues, G1 grade and G2 grade were 20%, 65%, 100% respectively. The difference between normal human bone tissues
and G1 grade, G2 grade were significant (P<0.05)respectively; the difference between G1 grade and G2 grade were significant(P<0.
05). Expression of stathmin in normal human bone tissues，G1 grade and G2 grade were (0.34±0.15）,（0.68±0.21）,（0.90±0.17）
respectively. The difference be- tween normal human bone tissues and G1 grade, G2 grade were significant (P<0.01) respectively; the
difference between G1 grade and G2 grade were significant (P<0.01). Conclusion: Overexpression of stathmin plays an important role in
development and progression of human osteosarcoma. |
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