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目的：比较分析高口气值组和低口气值组幼儿舌脱落细胞形态计量学参数及分类计数上的差异，探讨口气是否影响舌粘膜上皮细胞的角化、凋亡过程。方法：3-5 岁幼儿40 名，其中高口气值组幼儿20 名，低口气值组幼儿20 名，，取舌背中1/3 脱落细胞，制作细胞涂片、H.E 染色。应用计算机图像分析系统对舌脱落细胞进行形态计量学检测和分类计数。结果：高口气值组幼儿的舌脱落细胞的各类细胞的细胞形态较低口气值组幼儿小，且细胞核也更小。中层细胞、完全角化细胞计数在两组间的差异无统计学意义(P＞0.05)，高口气值组角化前细胞计数明显高于低口气值组(P＜0.01)，而高口气值组不全角化细胞计数明显低于低口气值组 (P＜0.01)。结论：高口气值组幼儿的舌脱落细胞较小，细胞角化程度较低，凋亡速度较慢。

英文摘要:

Objective: To compare cell morphometric parameters and classified amount of lingual exfoliated cell of high VSCs group and low VSCs group in children and study whether oral malodor affects the keratinization process of the lingual exfoliated cells. Methods: Forty children of 3-5 years old were enrolled in this study. According to their VSCs values, they were divided into high VSCs group and low VSCs group. Exfoliated cells samples from all the 40 children were obtained from the middle 1/3 area of dorsum of the tongue, then cell smears were made and stained with H.E. Cell morphometrically and classified amount study were made with computer image analysis system. Results: There were difference of morphometric parameters and classified amount between high VSCs group and low VSCs group. Lingual exfoliated cells of the children of high VSCs group were smaller with smaller nucleus than those of the children of low VSCs group. The proportion of middle layer cell and horny cell had no difference in these two groups (P＞0.05). The proportion of prekeratinocyte in high VSCs group was significantly higher than in low VSCs group (P＜0.01). The proportion of cornified-incompletely cell in high VSCs group was significantly lower than in low VSCs group (P＜0.01). Conclusion: Oral malodor can inhibit apoptosis process and depress keratinization degree of lingual exfoliated cells in children

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