| 伊璨1,2 谢伟东2 吕青1,2 张雅鸥2.miR-143 通过抑制PTN 促进人骨髓间充质干细胞成脂分化[J].现代生物医学进展英文版,2011,11(18):3401-3404. |
| miR-143 通过抑制PTN 促进人骨髓间充质干细胞成脂分化 |
| miR-143 Accelerates hMSCs Adipogenesis by Suppressingthe Expresssion of PTN |
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| DOI： |
| 中文关键词: miR-143 hPTN 成脂分化 hMSCs |
| 英文关键词: miR-143 hPTN Adipogenesis hMSCs |
| 基金项目:国家自然科学基金项目（30871428） |
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| 中文摘要: |
| 目的：研究miR-143 调控人骨髓间充质干细胞（hMSCs）成脂分化的新机理。方法：将NC、miR-143、siPTN、miR-143i 转入
hMSCs 中，诱导成脂分化，检测miR-143 对成脂分化的影响。经miRNA 靶点分析软件Findtar 预测出miR-143 在人多效生长因子
（hPTN） 的3'-UTR 端有靶点。RT-PCR、western blot 研究miR-143 与hPTN 的关系。构建hPTN 3'-UTR 靶位点荧光检测质粒
prltk-PTN 及其突变质粒prltk-m，验证miR-143 是否在人PTN 3'-UTR 上有靶点。结果：miR-143 促进hMSCs 成脂分化，抑制
hPTN 的mRNA 和蛋白表达水平。荧光报告实验证实miR-143 在人PTN 的3'-UTR 上有靶点。结论：miR-143 通过与hPTN
3'-UTR 上的靶点相结合而抑制hPTN 的表达，从而促进了hMSCs 成脂分化进程。 |
| 英文摘要: |
| Objective: To investigate the mechanism of miR-143 in the regulation of hMSCs adipogenesis. Methods: hMSCs were
transfected with NC/miR-143/siPTN/miR-143i and induced into adipogenic differentiation to detect the effect of miR-143 on adipogenesis.
The miR-143 target in 3'-UTR of hPTN was predicted by Findtar-software of miRNA. The relationship between miR-143 and hPTN
was researched by the methods of RT-PCR and western blot. The target of miR-143 predicted in hPTN 3'-UTR was verified by luciferase
expression of prltk-PTN plasmid inserted with hPTN 3'-UTR segment and prltk-m plasmid of mutant target. Results: miR-143 promoted
adipogenic differentiation of hMSCs, and suppressed the expression level of hPTN mRNA and protein. The experiment of luciferase
demonstrated that there was miR-143 target in hPTN 3'-UTR. Conclusion: miR-143 suppressed the expression of hPTN by combining
with target in hPTN 3'-UTR to accelerate the process of hMSCs adipogenesis. |
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