| 罗晖明1,2,3 康帅1 郑健△ 王强2 过立农1 林瑞超1.古尼虫草与亚香棒虫草亲缘关系初探[J].现代生物医学进展英文版,2011,11(17):3355-3358. |
| 古尼虫草与亚香棒虫草亲缘关系初探 |
| Preliminary Study on the Phylogenetic Relationship of Cordyceps Gunniiand Cordyceps Hawkesii |
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| DOI： |
| 中文关键词: 古尼虫草 亚香棒虫草 亲缘关系 ITS COI |
| 英文关键词: Cordyceps gunnii Cordyceps hawkesii Phylogenetic relationship ITS COI |
| 基金项目: |
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| 中文摘要: |
| 目的：用分子生物学方法，对古尼虫草和亚香棒虫草进行研究，对其寄主昆虫COI（cytochrome oxidase subunit I，细胞色素氧
化酶亚基I）和真菌ITS ( Internal Transcribed Sequence, 内转录间隔区)区的基因序列进行比较，以确定两者亲缘关系。方法：在古
尼虫草和亚香棒虫草性状研究的基础上，对两者来源真菌ITS 区和寄主昆虫COI 基因进行了PCR 扩增和序列测定，对序列进行
比对分析，并与GenBank 核酸序列数据库中的序列进行BLAST 检索比对。结果：发现古尼虫草和亚香棒虫草的来源真菌ITS 区
和寄主昆虫COI 基因序列均有较高相似度。结论：古尼虫草和亚香棒虫草有较近的亲缘关系。 |
| 英文摘要: |
| Objective: To determine the genetic relationship between Cordyceps gunnii and Cordyceps hawkesii, we conducted a
comparative study of the rDNA ITS (Internal Transcribed Sequence )regions of sources fungi and COI (cytochrome oxidase subunit I, cell
pigment oxidation enzyme subunits I ) gene of hostinsects. Methods: On the basis of properties research of Cordyceps gunnii and
Cordyceps hawkesii., The rDNA ITS (Internal Transcribed Sequence) regions of sources fungi and COI (cytochrome oxidase subunit I,
cell pigment oxidation enzyme subunits I ) gene of hostinsects were amplified and sequenced.The sequences were analyzed and
compared with sequences in Genbank database. Results: For sequences of rDNA ITS regions of sources fungi and COI gene of
hostinsects ,there are high similarities between Cordyceps gunnii and Cordyceps hawkesii. Conclusions: Close relationship exists in
Cordyceps gunnii and Cordyceps hawkesii. |
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