| 柳洁1 田海文1 谢正兰1 吴尚辉2 饶利兵.怀化医学高等专科学校医学形态学实验中心[J].现代生物医学进展英文版,2011,11(17):3262-3265. |
| 怀化医学高等专科学校医学形态学实验中心 |
| The Growth inhibitory Effects by Transfection of Anti-Oncogene P16on Human Hepatocarcinoma Cell Line |
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| DOI： |
| 中文关键词: 肝肿瘤 p16 基因 基因治疗 转染 脂质体 |
| 英文关键词: Hepatocellular neoplasm p16 gene Gene therapy Transfection Liposome |
| 基金项目:怀化市科学技术局科技计划立项项目（201019-25） |
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| 中文摘要: |
| 目的：探讨抑癌基因p16 对肝癌细胞生长的抑制作用及其机制。方法：将p16 cDNA 亚克隆至pcDNA3.1 真核表达载体上，
并经脂质体介导转染至人肝癌细胞株SMMC-7721。用MTT 法和Western blot 分析转染细胞的生长情况。结果：成功构建重组表
达质粒pcDNA3.1-p16，转染pcDNA3.1-p16 的SMMC-7721 细胞生长速度受到明显抑制；转染后有外源p16 蛋白的表达，且伴随
Bax 上调，Bcl-2 和cIAP2 的下调。结论：重组pcDNA3.1-p16 质粒能在人肝癌细胞SMMC-7721 内表达，且能抑制SMMC-7721
的生长，其机理与诱导肿瘤细胞凋亡相关。 |
| 英文摘要: |
| Objective: To investigate the inhibitory effect of tumor suppressor gene p16 on the growth of hepatocarcinoma cell.
Methods: p16 cDNA was subcloned into pcDNA3.1 eucaryotic expressing vector; then the recombinant pcDNA3.1-p16 plasmid was
transfected into human hepatocarcinoma cell line SMMC-7721 with liposome. MTT method and Western blot were employed to
investigate cell growth. Results: The recombinant plasmid pcDNA3.1-p16 was constructed successfully; the growth of hepatocarcinoma
cell line SMMC-7721 was obviously inhibited after transfection; the exogenous p16 protein was expressed, with the up-regulation of Bax
as well as down-regulation of Bcl-2 and cIAP2. Conclusion: The recombinant plasmid pcDNA3.1-p16 is able to express p16 protein in
human hepatocarcinoma cell line SMMC-7721 and suppress the cancer cell growth, which is associated with apoptosis. |
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