| 李俊杰刘亚△ 邱玉金田云虎李坤.持久脊柱负荷与椎间盘组织中MMP-2 表达关系的研究[J].现代生物医学进展英文版,2011,11(14):2730-2733. |
| 持久脊柱负荷与椎间盘组织中MMP-2 表达关系的研究 |
| Relationship Between Prolonged Spinal Loading and Expression ofMMP-2 in Intervertebral Discs |
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| DOI： |
| 中文关键词: 椎间盘退变 模型 生物力学 MMP-2 |
| 英文关键词: Disc degeneration Model Biomechanics MMP-2 |
| 基金项目: |
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| 中文摘要: |
| 目的：建立一压力可控型椎间盘退变模型，并探讨持久的脊柱负荷对椎间盘MMP-2 表达的影响。方法：选用54 只成年
Wistar 大鼠随机分为三组，分别模拟人类在站立(A 组1.12N)、坐位直立(B 组1.68N)、坐位前屈(C 组3.08N)三种状态下椎间盘内
的负荷情况，给予大鼠尾椎Co9/10 椎间盘恒定压力加压，以相邻Co8/9 椎间盘不加压作为对照（D 组）。三组分别在3、7、14 天后
取受压及对照椎间盘标本，进行HE 染色组织学观察及免疫组织化学分析，观察椎间盘退变情况及MMP-2 在椎间盘组织中的含
量变化。结果：随时间与压力的增加，椎间盘组织学评分与MMP-2 表达增高（P<0.05），MMP-2 表达与椎间盘退变程度成正相关
（r=0.870 ，P<0.05）。结论：持久的脊柱负荷可引起椎间盘退变及MMP-2 表达增加，MMP-2 可能在椎间盘退变的过程中发挥重要
作用。 |
| 英文摘要: |
| Objective: To establish an in vivo mouse model of compression-control degeneration and to investigate the effects of
prolonged mecanial loading on MMP2. Methods: A total of 54 adult Wistar rats were randomly divided into three groups, group A: the
pressure was 1.12N (simulating human pressure of the disc when human stand), group B: the pressure was1.68N (simulating human sitting),
group C: the pressure was 3.08N (simulating human sitting flexion). Group D: control group without any pressure to the disc. Static
compression was applied to mouse coccygeal discs in vivo for 3, 7, or 14 days. The expression of MMP-2 in four groups were detected by
immunohistochemical technique. Results: The degeneration of disc and the expression of MMP-2 increased with the increasing of the
time and pressure (P< 0.05), and a positive correlation was observed between the degeneration grade of disc and the amount of MMP-2
(r=0.870,P<0.05). Conclusion: Prolonged spinal loading cause the disc degeneration and the increasing expression of MMP-2, MMP-2
may be one of the important factors leading to intervertebral disc degeneration. |
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