| 李胜昔1 曾斌2△ 石巍2 廖爱军2 曹文涛2.姜黄素对肝癌细胞HepG2 的抗癌作用及P21WAF1/CIP1 表达的影响[J].现代生物医学进展英文版,2011,11(14):2658-2660. |
| 姜黄素对肝癌细胞HepG2 的抗癌作用及P21WAF1/CIP1 表达的影响 |
| Effects of Curcumin in Inhibition of Growth of Hepatoma HepG2 Cellsand Expression of P21WAF1/CIP1 |
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| DOI： |
| 中文关键词: 姜黄素 肝癌细胞 P21WAF1/CIP1 |
| 英文关键词: Curcumin Liver carcinoma cell P21WAF1/CIP1 |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨姜黄素对肝癌HepG2 细胞抗癌作用及相关周期蛋白依赖激酶抑制因子P21WAF1/CIP1 表达的影响。方法：体外培养肝
癌HepG2 细胞，用MTT 法检测姜黄素对HepG2 细胞的抑制作用，以RT-PCR 方法检测HepG2 细胞中P21WAF1/CIP1mRNA 的表达，
用免疫细胞化学检测其P21WAF1/CIP1 蛋白的表达。结果：姜黄素呈时间剂量性抑制HepG2 细胞的生长，并显著上调HepG2 细胞中
P21WAF1/CIP1mRNA 和蛋白的表达。结论：姜黄素能抑制HepG2 细胞的生长，并上调其中P21WAF1/CIP1 的表达。 |
| 英文摘要: |
| Objective: To study the anticancer activities of curcumin on human liver carcinoma cell line HepG2 and the effects of
express of P21WAF1/CIP1. Methods: Human liver carcinoma HepG2 cell line was cultured in vitro, proliferation inhibitory effect was tested by
MTT assay. The express of P21WAF1/CIP1 mRNA was determined by RT-PCR and the express of P21WAF1/CIP1 protein was messured by immunohistochemistry.
Result: The proliferation of HepG2 cells was inhibited by curcumin with dose and time-related effects, Curcumin
significantly improve the express of P21WAF1/CIP1 mRNA and protein. Conclusion: Curcumin can inhibit The proliferation of Human liver
carcinoma HepG2 cell line and improve the expressION of P21WAF1/CIP1 mRNA and protein. |
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