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目的：研究姜黄素对肺纤维化大鼠肺成纤维细胞增殖、凋亡的影响，探讨凋亡诱导因子（AIF）在肺成纤维细胞凋亡中的作用。方法：将体外培养的肺纤维化大鼠成纤维细胞，分别于不同浓度的姜黄素（5、10、20、40 μM）和caspase-3 抑制剂Z-DEVD-fmk （20 μM）孵育，观测细胞生长状态变化。MTT 检测成纤维细胞增殖抑制率；流式细胞仪检测细胞凋亡率；Western-Blot 测定凋亡诱导因子（AIF）蛋白表达及核转位结果：流式细胞术检测细胞凋亡，5～40 μM 姜黄素处理12 h，其凋亡率呈浓度依赖，对照组相比，差异显著；而抑制caspase-3 并不能完全阻止细胞凋亡。Western-Blot 结果显示，姜黄素处理组出现凋亡诱导因子（AIF）蛋白表达与核转位，抑制caspase-3 活性后未检测出AIF 表达结论：姜黄素可抑制肺成纤维细胞增殖，其诱导大鼠肺成纤维细胞凋亡，可能与线粒体释放AIF 有关。

英文摘要:

Objective: To the effect of curcumin on proliferation and apoptosis and the mechanism of apoptosis-inducing factor (AIF) on rat pulmonary fibroblasts. Methords: Fibroblasts derived from lung tissue of rat were cultured in vitro. Cultured cells were exposed to curcumin with different final concentration (5 μM,10 μM,20 μM,40 μM) and Z-DEVD-fmk with final concentration(20 μM). MTT assay was used to determine the inhibitory rate of curcumin on the proliferation of cells. Flow cytometry identified the apop- tosis. Western-blot was used to determine the expression and nuclear translocation of AIF protein. Results: Curcumin inhibited prolifera- tion of pulmonary fibroblasts in a dose-dependent manner (5 μM,10 μM,20 μM,40 μM), which was detected by Flow cytometry. But the proliferation inhibitory and the apoptosis induced by curcumin were not inhibited by Z-DEVD-fmk. In curcumin treated groups, AIF was observed associated with the nuclear translocation. Conclusion: Curcumin inhibited proliferation of pulmonary fibroblasts. The curcumin- induced apoptosis may be associated with the releasing of AIF from mitochondria.

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