| 张亮1 孙香蕾2 雷蕾1 李锋2 吴昌归1.吸烟大鼠肺脏AQP4 和MUC5AC 的表达及其与
一氧化氮的关系[J].现代生物医学进展英文版,2011,11(13):2455-2457. |
| 吸烟大鼠肺脏AQP4 和MUC5AC 的表达及其与
一氧化氮的关系 |
| Expression of AQP4 and MUC5AC in Lungs of Smoking Rats and ItsRelation with Nitric Oxide |
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| DOI： |
| 中文关键词: 水通道蛋白 一氧化氮 粘蛋白5AC 吸烟 |
| 英文关键词: Aquaporin Nitric oxide Mucusin 5AC Tobacco smoking |
| 基金项目:国家重点基础发展规划项目（973 项目）（2009CB522706） |
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| 中文摘要: |
| 目的：研究烟草烟雾吸入对大鼠肺组织水通道蛋白4(AQP4)和粘蛋白5AC(MUC5AC)表达的影响及其与支气管肺泡灌洗液
内一氧化氮代谢物水平的关系，探讨氧化应激对肺部水转运和粘液分泌的影响。方法：免疫组化法观察AQP4 在肺组织内的表
达, 平均光密度法比较模型组和空白组大鼠AQP4 的表达差异；半定量RT-PCR 法检测肺组织内AQP4 及MUC5AC mRNA 的表
达水平；硝酸还原酶法测定各组大鼠支气管肺泡灌洗液内一氧化氮代谢产物的浓度，分析模型组AQP4、MUC5AC mRNA 的表达
水平与支气管肺泡灌洗液内一氧化氮代谢物浓度之间的相关关系。结果:AQP4 在空白对照组呈强阳性染色，在模型组呈弱阳性
染色，两者平均光密度值有显著差异（P＜0.05）。模型组动物肺组织AQP4 mRNA 的表达降低，MUC5AC mRNA 的表达升高，与
空白组比较均有显著差异（P＜0.05），模型组动物支气管肺泡灌洗液内一氧化氮代谢产物的浓度与肺组织AQP4 mRNA 表达水平
呈负相关，相关系数r = -0.798,（P＜0.05），与MUC5AC mRNA 的表达水平呈正相关，相关系数r=0.857,（P＜0.05）。结论：吸烟可
导致肺组织AQP4 表达下降进而影响气道内水的转运。一氧化氮可能参与了烟雾吸入动物模型中AQP4 与MUC5AC 基因表达
的调控。 |
| 英文摘要: |
| Objective: To investigate the relationship of the expression of AQP4 and MUC5AC in tobacco-smoking and nitric
oxide metabolics in BALF, and to investigate the effect of oxidative stress on water transport of lung. Methods: Tobacco-smoking model
was established and the expression of AQP4 in lungs was examined by immunohistochemical stain and optical density analysis. AQP4
and MUC5AC mRNA expression was detected by semiquantitive reverse transcription-polymerase chain reaction(RT-PCR). Nitric oxide
(NO) metabolics in BALF was detected by nitrate reductase assay and its relationship with AQP4 and MUC5AC mRNA expression was
analyzed by Pearson's correlation. Results: There was strong positive expression of AQP4 in control group while it was a weak positive
stain in smoking model group. Optical density analysis showed that their difference is statistically significant （P＜0.01）. In smoking
model group the expression of AQP4 mRNA decreased and MUC5AC mRNA increased compared with that in the control group （P＜
0.01）. In smoking model group, concentration of NO metabolics from BALF is negatively correlated with AQP4 mRNA level（r=-0.849,
P＜0.01）and positively correlated with MUC5AC mRNA level （r=0.857, P＜0.01）Conclusions: Smoking causes the decreasing of
AQP4 expression in lung tissue which influences water transport in airways. NO may play a vital role in this process through regulating
AQP4and MUC5AC mRNA expression. |
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