| 樊志强庞炜△ 杨连甲文艳华王海鹏张小平周勇范清宇.白介素1 受体拮抗剂及转化生长因子β1 对兔膝关节骨性关节炎（OA）
的治疗研究[J].现代生物医学进展英文版,2011,11(13):2447-2450. |
| 白介素1 受体拮抗剂及转化生长因子β1 对兔膝关节骨性关节炎（OA）
的治疗研究 |
| Interleukin-1 Receptor Antagonist and Transforming Growth Factor-β1for Rabbits with Osteoarthritis |
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| DOI： |
| 中文关键词: 骨性关节炎 白介素受体拮抗剂 转化生长因子 关节软骨 |
| 英文关键词: Osteoarthritis IL-1Ra TGF Articular cartilage |
| 基金项目:军队医药卫生科技项目（LXH-2006010） |
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| 中文摘要: |
| 目的：通过关节腔内注射白介素1 受体拮抗剂（IL-1Ra）及转化生长因子β1（TGF-β1），观察其对骨性关节炎的治疗作用。方
法：16 只3-4 个月龄新西兰大白兔（雌雄不限）随机分为四组，每组四只，分为正常组，对照组，IL-1Ra 组，TGF-β1 组，正常组仅打
开左膝关节关节腔，剩余三组均给予左膝关节前交叉韧带离断术（anterior cruciate ligament transaction ACLT）。四周后IL-1Ra 组
给予关节腔内注射IL-1 Ra 5g/ml，TGF-β1 组给予关节腔内注射TGF-β1 50mg/ml ，每周两次，连续四周，正常组及对照组未采取任
何措施。术后八周时采用空气栓塞法处死全部实验动物，取股骨远端长约1cm，固定后切片行细胞形态学观察。结果：大体肉眼观
察：对照组股骨远端软骨面色颜色变暗并稍显粗糙，可见少量软骨脱落，其余两组关节软骨大体形态均接近正常。镜下观察：
TGF-β1 组软骨细胞增生明显，软骨细胞排列较正常软骨细胞排列轻度紊乱。IL-1Ra 组软骨细胞增生数量未及TGF-β1 组明显，软
骨细胞排列也不及TGF-β1 组规则。IL-1Ra 组及TGF-β1 组软骨细胞形态接近正常软骨细胞，基质染色接近正常。两组软骨膜表面
光滑，无纤维细胞形成，软骨膜下未见纤维化。对照组软骨细胞数量明显减少且排列紊乱，软骨细胞形态发生改变，可见软骨细胞
凋亡，镜下可见软骨细胞核浓缩、裂解，并可见新生软骨形成，突出于正常软骨膜表面，软骨膜表面下方可见血管翳及纤维母细胞
形成，出现轻度纤维化。基质染色不均匀且染色较淡。结论：IL-1Ra 及TGF-β1 关节腔内注射均能有效抑制骨性关节炎关节软骨
的退变并增加软骨细胞再生，促进软骨基质合成，延缓骨性关节炎的发病进程。 |
| 英文摘要: |
| Objective: To observation the treatment of rabbit osteoarthritis by vivo interleukin-1 receptor antagonist (IL-1Ra) and
transforming growth factor-β1 (TGF-β1). Methods: A total of 16 three to four- month -old New Zealand white rabbits were divided
randomly into four experimental groups. Group one (normal group) received only opened the left articular cavity.Those in another three
groups(IL-1Ra group,TGF-β1 group and control group) received anterior cruciate ligament transection (ACLT).Four weeks later, IL-1Ra
group,TGF-β1 group were given vivo interleukin-1 receptor antagonist and transforming growth factor-β1 twice one week and anotheor
groups did nonthing.All animals were scarificed eight weeks later after surgery.The samples were stained for tissue slice. Results: In
general visual study IL-1Ragroup, TGF-β1 group and control group the articula cartilage close to normal. Control group cartilage is
stramineous and can find amotic. Observation by microscope: in IL-1Ragroup, TGF-β1 group and control group cartilage cell
proliferation obviously and arrange orderly, the control group cartilage cell decreased and choas and can find fibroplasia.Conclusion: By
vivo interleukin-1 receptor antagonist (IL-1Ra) transforming growth factor-β1 (TGFβ1) can effectively inhibit degeneration of articular
cartilage cell increase articular cartilage cell regeneration avilably.This will release clinical symptoms so to delay the process of
osteoarthritis. |
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