| 练海东1 任兵2△ 高晓唯2 郭继华3 解玉军4 张改丽4 赵旭东4 刘莹4.米诺环素对糖尿病大鼠视网膜神经细胞凋亡的影响[J].现代生物医学进展英文版,2011,11(13):2435-2437. |
| 米诺环素对糖尿病大鼠视网膜神经细胞凋亡的影响 |
| Effects of Minocycline on Apoptosis of Retinal Nerve Cells of Rats withDiabetic Retinopathy |
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| DOI： |
| 中文关键词: 糖尿病视网膜病变 米诺环素 凋亡 |
| 英文关键词: Diabetic retinopathy Mincycline Apoptosis |
| 基金项目: |
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| 中文摘要: |
| 目的：观察米诺环素对糖尿病大鼠视网膜神经细胞的凋亡的影响，研究米诺环素对糖尿病视网膜神经保护作用，对米诺环
素在糖尿病视网膜疾病中抑制神经细胞凋亡提供理论支持。方法：选择健康成年雄性SD 大鼠30 只,随机分成正常对照组、糖尿
病模型组和米诺环素治疗组,每组10 只。腹腔内注射链脲佐菌素(STZ) 诱发大鼠糖尿病。米诺环素治疗组给予米诺环素腹腔注射
（45mg/kg）,共注射10d,模型组和对照组腹腔注射等体积生理盐水, 于给药后8 周的3 组动物处死，冰浴下取其视网膜组织，随后
制备视网膜石蜡切片,采用末端脱氧核糖核酸介导生物素化脱氧尿嘧啶缺口末端标记(TUNEL)法进行凋亡细胞原位标记,行视网
膜神经细胞凋亡计数,对所有数据进行统计学分析。实验结果拟用均数和标准差（x±s）表示，P＜0.05 为差异有统计学意义。结果：
相同观察时相，与阴性对照组比较, 模型对照组大鼠视网膜TUNEL 阳性细胞显著增多(P＜0.01) , 在米诺环素组, 大鼠视网膜
TUNEL 阳性细胞数比模型对照组明显减少,差异有统计学意义(P＜ 0.01)。结论：米诺环素能有效降低糖尿病大鼠视网膜神经细
胞的凋亡，对糖尿病视网膜神经细胞有保护作用。 |
| 英文摘要: |
| Objective: To study the effect of the Minocycline （MNC）on expression of apoptosis in retinal nerve cells of rat with
diabetic retinopathy（DR）. Methods: A total of 30 male SD rats were randomly divided into negative control group,model control group
and MNC treated group. Diabetes model was established by intraperitoneal injection of 60 mg/kg streptozotocin (STZ). Terminal
Deoxynucleotidyl Transferase Biotin-dUTP Nick End Labeling (TUNEL staining) was used to observe neuron apoptosis in retina. All the
experimental data were expressed by Mean+standard deviation. Results: Compared with the negative control group , TUNEL positive
cells in retina were increased significantly (P＜0.01) in model control group. However, TUNEL positive cells in retina in MNC treated
group were significantly decreased ( P＜0.01). Conclusion: MNC can obviously decrease the expression of apoptosis in retina with DR.
It is one of path of inhibiting impairment on retinal nerve cells with DR. |
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