| 王清莹姜飞洲庄玉玉万小平.白藜芦醇对人子宫内膜癌细胞系AN3CA 增殖和凋亡效应
及其机制探讨[J].现代生物医学进展英文版,2011,11(13):2401-2404. |
| 白藜芦醇对人子宫内膜癌细胞系AN3CA 增殖和凋亡效应
及其机制探讨 |
| Effects of Resveratrol on Proliferation and Apoptosis of EndometrialCarcinoma AN3CA Cells and Its Mechanism |
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| DOI： |
| 中文关键词: 白藜芦醇 子宫内膜癌 ERK1/2 通路 增殖 凋亡 |
| 英文关键词: Resveratrol Endometrial carcinoma Proliferation Extracellular regulated kinase1 /2 Apoptosis |
| 基金项目:国家自然科学基金资助项目（81072139） |
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| 中文摘要: |
| 目的：探讨白藜芦醇(resveratrol，Res)对人子宫内膜癌细胞AN3CA 的增殖抑制和凋亡诱导效应及可能存在的机制。方法: 应
用噻唑蓝(MTT)法检测Res 对AN3CA 的增殖影响；流式细胞术检测Res 对细胞周期分布和凋亡影响；荧光实时定量PCR 检测
Res 对细胞Bcl-2、Bax 和MMP-9mRNA 表达水平的影响；Western Blot 方法检测Res 对PCNA、Bcl-2、Bax 及ERK1/2、p-ERK1/2
蛋白表达水平的影响。结果: Res 对子宫内膜癌细胞AN3CA 具有显著的生长抑制作用（P＜0.01），呈时间- 剂量依赖性；不同浓度
Res 处理细胞G0/G1 期比例显著增加伴随S 期细胞数的减少；细胞凋亡率明显增高，200μmol/l Res 处理48h 凋亡率可达30.96%
±2.041%（P ＜0.01）。与对照组相比，Res 能抑制PCNA 的蛋白表达量，增加Bax 和降低Bcl-2 转录和蛋白水平的表达量。Res 在短
时间内（0.5-1h）激活ERK1/2 的磷酸化表达但随着作用时间延长（4-48h）其表现为抑制效应。结论: Res 具有抑制AN3CA 细胞增
殖，诱导细胞G0/G1 期阻滞和凋亡的效应。Res 诱导凋亡可能是通过上调Bax，下调Bcl-2 发挥作用，其抗癌作用机制可能与
ERK1/2 通路失调相关。 |
| 英文摘要: |
| Objective: To investigate the effects of resveratrol (Res) on proliferation and apoptosis of endometrial cancer cell lines
AN3CA and explore the involved mechanism in vitro. Methods: AN3CA cells were treated with different concentrations of resveratrol
for various periods. The cell growth inhibitory rate was examined by MTT assay. The cell cycle arrest and apoptosis induction of
resveratrol in AN3CA cells were detected by flow-cytometry analysis.We utilized real-time quantitative polymerase chain reaction(PCR)
to identify expression level of Bcl-2,Bax and MMP-9 mRNA.The protein expression level of PCNA,Bcl-2,Bax,ERK1/2 and p-ERK1/2
were detected by Western-blot.Results: Resveratrol has significantly anti-proliferation effect on AN3CA cells which is in time- and
concentration-dependent manners. It increases the G0/G1 phase cell population, whereas a concomitant decrease of cell numbers in S
phase(P ＜0.01). In addition, resveratrol treatment could induce cellular apoptosis that the rate of apoptosis increased to 30.96%±2.041%
(200μmol/l Res).Compared with the control, resveratrol treatment caused a decrease in the levels of PCNA and Bcl-2,whereas the level of
Bax expression was upregulated. In a short time (0.5-1h)，resveratrol can activate ERK1/2 phosphorylation but with the time prolonged, it
showed inhibition of the expression of p-ERK. Conclusion: It seems that resveratrol has the antitumor action including inhibition of cell
proliferation, blocking cells at the G0/G1 phase and inducing apoptosis. It regulated expression level of apoptosis-related Bcl-2 family
protein. The antitumor effect of resveratrol is possiblly mediated by ERK1/2 pathway. |
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