高飞艳1 刘会玲2 杨燕3 祝秉东3△ 杨毓琴2.生物可降解微球作为HPV-E7 蛋白免疫佐剂的研究[J].现代生物医学进展英文版,2011,11(12):2211-2214. |
生物可降解微球作为HPV-E7 蛋白免疫佐剂的研究 |
Research on Biodegradable Microspheres as HPV-E7 Protein Adjuvant |
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DOI: |
中文关键词: HPV-E7 蛋白 微球 PLGA 铝佐剂;DDA |
英文关键词: HPV E7 protein Microsphere PLGA Al(OH)3 DDA |
基金项目:兰州大学功能有机分子化学国家重点实验室资助 |
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中文摘要: |
目的:对聚乳酸聚羟基乙酸(PGLA)作为疫苗运输载体进行免疫学评价。方法:用复乳法制备PLGA 微球,通过表面吸附人乳
头状瘤病毒(HPV) E7 蛋白制备成聚乳酸聚羟基乙酸(PGLA)微球,考察粒径分布情况及体外释放水平,通过皮下免疫注射途径免
疫C57BL/6 小鼠,用间接ELISA 法检测免疫鼠血清中的抗体水平,由此评价PLGA 微球疫苗运输载体的佐剂效应。。结果:复乳
法制备的PLGA 微球表面光滑,大小均匀,包封率20.1%,注射小鼠6 周后(第2 周加强免疫1 次),微球疫苗诱导产生的IgG1 抗
体水平较同剂量的铝佐剂组和溴化二甲基双十八胺(DDA)组明显升高,(平均滴度分别为3805、1270、2262);微球疫苗诱导产生
IgG2b 的抗体水平明显高于铝佐剂组,略低于DDA 组,(平均滴度分别为1131、475、2653) 而IgG2c 的抗体量高于铝佐剂组和
DDA 组(平均滴度分别为150、36、106)。结论:人乳头状瘤病毒E7 蛋白聚乳酸羟基乙酸微球作为疫苗输送体系可以明显的提高
抗原的免疫原性。 |
英文摘要: |
Objective: To evaluate the immune response of PLGA-MS used as carrier to carry the vaccinum. Methods: Microspheres
were prepared by double--emulsion solvent evaporation method. HPV E7 protein was used an antign absorb different adjuvant
conclude aluminum hydroxide hydrate [Al (OH)3], Dimethyl dioctadecylammonium (DDA) and poly (lactic-co-glycolic) acid (PLGA)
microspheres to immunize C57BL/ 6 mouse with the subcutaneous administration. The IgG1、IgG2b and IgG2c were detected by indirect
ELISA. Result: The microspheres had good shape with smooth and uniform surface, and the mean particle size were about 1 um and
surface associated protein were 20.1%. 6 weeks later, IgG1 antibody levels in C57BL/ 6 mouse injected by E7 protein of HPV-loaded
PLGA microsphere was higher than those injected with E7 protein of HPV puls Al (OH)3 and DDA (the midpointtiters were
3805,1270,2262, respectively); IgG2b antibody levels was significantly higher than that of Al (OH)3 and slightly less than that of DDA
(the midpointtiters were 1131,475,2653 respectively);In terms of the IgG2b antibody levels, It is higher than that of Al(OH)3 and DDA
(the midpointtiters were 150, 36, 106 respectively). Conclusion: Biodegradable PLGA microsphere was an effective system for conteolled
delivery of vaccines. |
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