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目的：探讨宫颈组织p53 基因第72 位密码子的多态性及分析第72 位密码子的多态性与湖南地区汉族人群宫颈鳞癌的相关性。方法：采用PCR 方法扩增101 例正常宫颈和150 例宫颈鳞癌石蜡组织p53 基因第72 位密码子基因，回收目的片段进行测序。采用SPSS 11.5 软件分析p53 基因第72 位密码子的多态性。结果：p53 第72 位密码子基因测序结果显示，在宫颈鳞癌组织中 Arg/Arg、Pro/Pro、Arg/Pro 所占比例分别为40.66%、16.67%、42.67%；在正常宫颈组织中Arg/Arg、Pro/Pro、Arg/Pro 所占比例分别为 47.53%、7.92%、44.55%。统计学分析结果显示，Arg/Arg 和Arg/Pro 基因型在宫颈鳞癌和对照组中的表达差异没有统计学意义 (P>0.05)；Pro/Pro 基因型在宫颈鳞癌组中所占比例显著高于正常宫颈组织(P<0.05)。结论：p53 基因第72 位密码子Pro/pro 基因型是湖南地区女性发生宫颈鳞癌易感因素。

英文摘要:

Objective: To study the p53codon72 polymorphism of cervical tissues, and analysis the relationship between p53codon72 polymorphism and cervical carcinoma of Han population in Hunan area. Methods: Use PCR to amplify the p53codon72 of 101 normal cervical and 150 cervical carcinomas paraffin tissues, purifying these fragments and sequencing. Used SPSS 14.0 software to analysis the p53codon72 polymorphism. Results: The sequencing results of p53codon72 showed that in cervical carcinoma tissues, the proportion of Arg/Arg, Pro/Pro, Arg/Pro was 31.33%, 59.33%, 9.33% respectively; in normal cervical tissue, the proportion of Arg/Arg, Pro/Pro, Arg/Pro was 42.57%, 54.46%; 2.97% respectively. Statistical analysis showed that the Arg/Arg and Arg/Pro genotype expression difference was not statistically significant (P>0.05) between cervical carcinomas tissues and normal cervical tissues; the Pro/Pro genotype expression in cervical carcinomas tissues was significantly higher than the normal cervical tissues (P<0.05). Conclusion: p53codon72 Pro/Pro genotype is one of the susceptibility factors to cervical carcinomas in Han women in Hunan area.

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