| 汪宇1 王丽岩2△ 贺立新2 马瑞风3.钙网质蛋白在多囊卵巢综合征大鼠子宫内膜的表达及意义[J].现代生物医学进展英文版,2011,11(11):2075-2078. |
| 钙网质蛋白在多囊卵巢综合征大鼠子宫内膜的表达及意义 |
| Expression and Significance of Calreticulin in Endometrium of Rats withPolycystic Ovarian Syndrome |
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| DOI： |
| 中文关键词: 多囊卵巢综合征 子宫内膜 动物模型 钙网质蛋白 |
| 英文关键词: PCOS Endometrium Animal model Calreticulin |
| 基金项目:Expression and Significance of Calreticulin in Endometrium of Rats with
Polycystic Ovarian Syndrome |
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| 中文摘要: |
| 目的：研究钙网质蛋白（CRT）在PCOS 大鼠子宫内膜中的表达及生物学意义。方法：60 只雌性SD 大鼠随机分为PCOS 组和
对照组,每组各30 只。给模型组24 日龄大鼠皮下埋植左旋甲基炔诺酮硅胶棒3 mm / 只, 3 d 后BID 皮下注射人绒毛膜促性腺激
素1.5 I U。给对照组皮下注射等体积生理盐水。注射9 d 后观察大鼠卵巢形态学(HE 染色) ,化学发光法测定性激素水平。结果：
模型组大鼠卵巢重量和体积均显著高于对照组(P<0.01)。模型组大鼠卵巢出现类多囊卵巢综合征的改变。模型组卵巢各级发育期
卵泡及黄体少见,卵泡多呈囊性扩张。模型组大鼠血清孕激素、睾酮、空腹胰岛素、空腹血糖水平均显著高于对照组(P< 0.05); 卵
泡刺激素（FSH）水平显著低于对照组(P<0.05)。LH / FSH 比值显著高于对照组(P< 0.05)。采用免疫组织化学方法及灰度值测定,
定量分析CRT 在PCOS 组和对照组的子宫内膜中表达。CRT 在两组中的子宫内膜中均有表达。PCOS 组子宫内膜上皮CRT 表
达显著低于对照组(P< 0.01)。结论：避孕硅胶棒联合hCG 诱导SD 大鼠多囊卵巢综合征模型是较好的PCOS 模型。CRT 与PCOS
的发病密切相关。 |
| 英文摘要: |
| Objective: To investigate the expression and biological significance of apoptosis regulatory CRT in the follicles and
endometrium of polycystic ovarian syndrome (PCOS) in rat model. Methods: Sixty rats were randomly divided into two groups: PCOS
group (n=30) and control group (n= 30). The rats in the experimental group were given subcutaneous implanting of levonorgestrel silica
gel staff and injected 115 I U hCG twice daily for 9 days from the 4th day. The rats in the control group were injected with normal saline
at the same time. Ovarian morphologic changes, sex hormone levels were detected. Results: The weight and volume of ovarian in the
model rats were higher than that in the control rats′(P<0.01). The rats in model group had similar pathological appearance to polycystic
ovary. The multiple follicular cysts, and the number of thecacells and interstitial cells increased in the ovaries of modeling group showed .
There was less developed follicles and corpus lutea. The progesterone, testosterone, luteinizing hormone, fasting insulin, glucose the LH
/FSH ratio were significantly higher in experimental group than those in control group, so as. There was the expression of apoptosis regulatory
protein CRT. The CRT protein expressed in endometrium in PCOS group and control group. The expression of CRT of endometrial
in PCOS rats were significantly lower (P < 0.01) than those in control group. Conclusion: The rat model induced by levonorgestrel with
hCG was proved to be an ideal PCOS animal model. CRT may be closely correlated with the pathogenesis of PCOS. |
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