| 沈霞谭从娥陈莹冯居君张鑫.小鼠中血清因子结合位点位置分布及保守性的生物信息学分析[J].现代生物医学进展英文版,2011,11(11):2041-2044. |
| 小鼠中血清因子结合位点位置分布及保守性的生物信息学分析 |
| Bioinformatics Analysis of Position Distribution and Conservation of SerumResponse Factor Binding Site in Mice |
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| DOI： |
| 中文关键词: 血清因子结合位点 CArG 元件 生物信息学 替换率 位置分布 |
| 英文关键词: Serum response factor CArG elements Bioinformatics Substitution Position distribution |
| 基金项目:国家自然科学基金（NO:81072731） |
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| 中文摘要: |
| 目的：血清反应因子在与心血管相关的疾病基因调控方面的作用越来越重要。血清反应因子识别的结合位点CArG 元件因
其重要的基因调控作用近年来随之备受关。本研究目的是揭示血清因子结合位点的位置分布与功能的关系及CArG 元件内部各
个位点的保守性。方法：本研究应用生物信息学方法结合遗传学方法对小鼠中CArG 元件的位置分布、位点替换率及GO 分类进
行深入研究。结果：结果表明，71%的功能CArG 元件分布在转录起始位点上游，且距离转录起始位点越近，CArG 元件的数量越
多。保守性分析发掘出元件内部的替换冷点、热点及替换规律。GO 分类结果显示，CArG 依赖性基因多为信号转导和细胞骨架蛋
白。结论：上述研究结果将为准确预测候选CArG 元件提供重要理论基础，同时也将为更为深入阐述SRF 的调控模式奠定基础。 |
| 英文摘要: |
| Objective: The serum response factor is one of the valuable transcription factors because of its important role in gene
expression. In turn, cis-elements CArG bound by serum response factor is getting more and more attention. This study is going to reveal
the position distribution and conservation of CArG elements. Methods: We study the position distribution, substitution of CArG elements
and GO of CArG dependent genes in mouse by the bioinformatics and genetic methods. Results: Our results show that 71% functional
CArG elements exist upstream to the annotated TSS, with copy number increasing as one move closer to the TSS. The conservation analysis
revealed the substitution pattern. What' more, GO analysis results show that the CArG-dependent genes mostly are signaling and cytoskeleton
protein. Conclusion: The results will be a great help in predicting the candidate CArG elements and attempting to distinguish
the functional CArG elements through alignments. And it will be fundamental in study the regulatory system of SRF. |
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