| 柴立民1，2 张园2 车永哲2 杨荣存2.小鼠富亮氨酸重复结构蛋白Lrig2 基因、蛋白结构及组织定位分析[J].现代生物医学进展英文版,2011,11(11):2014-2017. |
| 小鼠富亮氨酸重复结构蛋白Lrig2 基因、蛋白结构及组织定位分析 |
| Structure and Tissue Localization Analysis of Mus Lrig2 Gene and Protein |
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| DOI： |
| 中文关键词: Lrr- 免疫球蛋白结构域样蛋白2 基因与蛋白结构 组织定位 |
| 英文关键词: Mus Leucine-rich repeat immunoglobulin-like domain protein 2 Gene and protein structure Tissues localization |
| 基金项目:国家高科技术研究发展计划（863 计划，No.2006AA020502） |
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| 中文摘要: |
| 目的：分析小鼠富亮氨酸重复结构蛋白家族成员Lrig2 的基因与蛋白的结构，明确其组织分布和定位，并对其功能进行初步
预测。方法：利用生物信息学分析技术对小鼠Lrig2 基因的染色体定位、蛋白结构进行分析预测；通过RT-PCR、mRNA 原位杂交
技术检测Lrig2 基因在小鼠不同组织中的表达定位；通过系统进化树分析Lrig2 与其他Lrrs 蛋白家族成员的同源性。结果：生物信
息学分析显示，Lrig2 是一种跨膜蛋白受体，是Lrrs 蛋白超家族成员之一，胞外区含有15 个Lrr 模序、3 个免疫球蛋白样结构域，
存在单一的跨膜结构域；Lrig2 在小鼠的多个组织中表达，其中在胸腺、脾脏等组织中表达较强；系统树分析显示，Lrigs 蛋白是
sLrPs 超家族成员，是一种跨膜蛋白受体。结论：Lrig2 在免疫组织中表达较强，推测其可能在肿瘤免疫应答进程中发挥重要的效
能。 |
| 英文摘要: |
| Objective: To investigate the gene and protein structure of Mus Leucine-rich repeat immunoglobulin-like domain protein
2 (Lrig2), and to definite its tissue distribution and localization, predict its biologic function. Methods: Bioinformatics analytical technique
was used to analyze the chromosomal localization and protein structure of Mus Lrig2. The expression distribution of Lrig2 in
mouse tissues was detected by RT-PCR and mRNA in situ hybridization. The phyletic evolution tree was used to analyze the homologous
with Lrrs protein family. Results: Lrig2 was a transmembrane protein, which was one member of Lrr protein family. There were 15 Lrr
motif and 3 immunoglobulin-like domains. This protein has one transmembrane domain. Lrig2 gene was expressed in several tissues,
especially in thymus and spleen. Lrigs proteins belong to a new class of the sLrP family. It is a transmembrane protein. Conclutions: The
expression of lrig2 in immunity tissues were notably, which indicated that Lrig2 played important biologic function in immunologic
response of cancer. |
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