柴立民1,2 张园2 车永哲2 杨荣存2.小鼠富亮氨酸重复结构蛋白Lrig2 基因、蛋白结构及组织定位分析[J].现代生物医学进展英文版,2011,11(11):2014-2017. |
小鼠富亮氨酸重复结构蛋白Lrig2 基因、蛋白结构及组织定位分析 |
Structure and Tissue Localization Analysis of Mus Lrig2 Gene and Protein |
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DOI: |
中文关键词: Lrr- 免疫球蛋白结构域样蛋白2 基因与蛋白结构 组织定位 |
英文关键词: Mus Leucine-rich repeat immunoglobulin-like domain protein 2 Gene and protein structure Tissues localization |
基金项目:国家高科技术研究发展计划(863 计划,No.2006AA020502) |
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中文摘要: |
目的:分析小鼠富亮氨酸重复结构蛋白家族成员Lrig2 的基因与蛋白的结构,明确其组织分布和定位,并对其功能进行初步
预测。方法:利用生物信息学分析技术对小鼠Lrig2 基因的染色体定位、蛋白结构进行分析预测;通过RT-PCR、mRNA 原位杂交
技术检测Lrig2 基因在小鼠不同组织中的表达定位;通过系统进化树分析Lrig2 与其他Lrrs 蛋白家族成员的同源性。结果:生物信
息学分析显示,Lrig2 是一种跨膜蛋白受体,是Lrrs 蛋白超家族成员之一,胞外区含有15 个Lrr 模序、3 个免疫球蛋白样结构域,
存在单一的跨膜结构域;Lrig2 在小鼠的多个组织中表达,其中在胸腺、脾脏等组织中表达较强;系统树分析显示,Lrigs 蛋白是
sLrPs 超家族成员,是一种跨膜蛋白受体。结论:Lrig2 在免疫组织中表达较强,推测其可能在肿瘤免疫应答进程中发挥重要的效
能。 |
英文摘要: |
Objective: To investigate the gene and protein structure of Mus Leucine-rich repeat immunoglobulin-like domain protein
2 (Lrig2), and to definite its tissue distribution and localization, predict its biologic function. Methods: Bioinformatics analytical technique
was used to analyze the chromosomal localization and protein structure of Mus Lrig2. The expression distribution of Lrig2 in
mouse tissues was detected by RT-PCR and mRNA in situ hybridization. The phyletic evolution tree was used to analyze the homologous
with Lrrs protein family. Results: Lrig2 was a transmembrane protein, which was one member of Lrr protein family. There were 15 Lrr
motif and 3 immunoglobulin-like domains. This protein has one transmembrane domain. Lrig2 gene was expressed in several tissues,
especially in thymus and spleen. Lrigs proteins belong to a new class of the sLrP family. It is a transmembrane protein. Conclutions: The
expression of lrig2 in immunity tissues were notably, which indicated that Lrig2 played important biologic function in immunologic
response of cancer. |
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