| 陈桂明1 尹路2△ 周光文1 施敏敏2 颜召文3.硝普钠灌注对移植小肠粘膜细胞凋亡的影响[J].现代生物医学进展英文版,2011,11(11):2011-2013. |
| 硝普钠灌注对移植小肠粘膜细胞凋亡的影响 |
| Effects of Sodium Nitroprusside on Mucosal Epithelium Apoptosisin Rats after Small Bowel Transplantation |
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| DOI： |
| 中文关键词: 小肠移植 粘膜屏障 细胞凋亡 硝普钠 |
| 英文关键词: Small bowel transplantation Mucosal barrier Apoptosis Sodium nitroprusside |
| 基金项目:国家自然科学基金资助项目(30671972) |
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| 中文摘要: |
| 目的：探讨外源性一氧化氮（nitric oxide, NO）供体硝普钠（sodium nitroprusside, SNP）对移植小肠粘膜细胞凋亡的影响。方
法：64 只220～300g 雄性SD 大鼠随机分成3 组：A1 组（n=8），仅行剖腹关腹手术；A2 组（n=12）：12 对大鼠随机作为供受体行同
种异体节段小肠移植，无SNP 干预；A3 组（n=16）：16 对大鼠随机作为供受体行同种异体节段小肠移植，SNP 加入灌注液进行供
肠灌注。采用前述3 组动物模型再灌注5 小时肠造口标本，TUNEL 法检测小肠蜡块标本的细胞凋亡情况。结果：与A1 组(3.86±
4.74%)相比，A2(22.44±10.94%)、A3 组(17.12±8.44%)小肠粘膜的细胞凋亡指数均有显著增高(P<0.05)，A3 组较A2 组细胞凋亡
指数显著降低(P<0.05)。结论：小肠移植导致小肠粘膜细胞凋亡增加，外源性NO 供体SNP 灌注能够显著降低植入小肠的细胞凋
亡，从而可能减弱粘膜屏障的损伤。 |
| 英文摘要: |
| Objective: To investigate the effect of sodium nitroprusside (SNP) treatment on mucosal epithelium apoptosis of the
transplanted intestine in rat. Methods: Sixty-four male Sprague-Dawley (SD) rats weighing 220~300g were randomly divided into three
groups. Group A1 (n=8): only performed laparotomy. Group A2 (n=12): 24 rats were randomly assigned to be donor or recipient to establish
small bowel transplantation (SBT) model, but no SNP treatment. Group A3 (n=16), 32 rats were randomly assigned to be donor or recipient
to establish SBT model, flushed with SNP when harvesting the graft. Harvested intestine samples from stoma 5h after reperfusion.
Detected apoptosis of transplanted intestine by TUNEL method. Results: There is higher apoptosis index of mucosal epithelial cells of
transplanted intestine in group A2(22.44±10.94%) and A3(17.12±8.44%) than that in A1(3.86±4.74%) (P<0.05). Fewer cells apoptosis
was found in group A3 (SNP flushed group) compared with A2. Conclusion: SNP used as a flushing solution element may attenuate
the mucosal barrier injury of transplanted intestine by depressing the mucosal cells apoptosis significantly. |
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