| 赵名艳李立华△ 周长忍周炬光.载荷壳聚糖微球的海藻酸钠水凝胶的制备[J].现代生物医学进展英文版,2011,11(11):2006-2010. |
| 载荷壳聚糖微球的海藻酸钠水凝胶的制备 |
| Preparation of Sodium Alginate Hydrogel Loaded With ChitosanMicrosphere |
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| DOI： |
| 中文关键词: 壳聚糖微球 溶菌酶 海藻酸钠水凝胶 缓释控释系统 |
| 英文关键词: Chitosan microsphere Lysozyme Sodium alginate hydrogel Controlled-release system |
| 基金项目:国家自然科学基金项目（30900307）；教育部博士点基金项目（200805590001）；广东省高等学校科技创新重点项目(CXZD1015) |
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| 中文摘要: |
| 目的：在支架材料上引入具有控释行为的微球，旨在通过微球包裹生长因子，通过生长因子的缓慢释放从而促进种子细胞
的生长分化。方法：本实验通过在海藻酸钠水凝胶中负载具有控释功能的壳聚糖微球，并通过在微球中包载溶菌酶从而达到控制
壳聚糖降解速率的功效。实验研究了不同搅拌速度下壳聚糖微球的形貌及粒径大小，通过扫描电镜对壳聚糖微球及复合支架的
形貌进行了观察，通过紫外光吸收法测试了微球的载药量及包封率，并研究了壳聚糖微球在体外的降解行为等。结果：制备的壳
聚糖微球表面较光滑，溶菌酶的包封率在25.78%-41.89%之间，载药量在15.20%-24.44%之间。包封溶菌酶的微胶囊在降解9 天后
壳聚糖分子量下降了70.40％，载荷微球的复合凝胶孔洞增多，孔洞大小均匀。结论：此复合材料有望作为载荷软骨相关生长因子
的支架模型，从而解决软骨组织工程中种子细胞匮乏的问题。 |
| 英文摘要: |
| Objective: Microspheres with sustained release have been introduced into scaffolds to enclose the growth factors, by
the slow-release of the growth factors to promote the growth and differentiation of seeded cells. In this study, Sodium alginate hydrogel
integrated with sustained release chitosan microsphere has been prepared and lysozyme was introduced into the chitosan microsphere to
control the degradation of chitosan. Methods: The research studied the morphology and grain sizes of the microsphere under different
stirring speed. The morphology of the microsphere morphology and grain sizes of the microsphere under different stirring speed. The
morphology of the microsphere and composite scaffolds were characterized with SEM, the drug loading rate and envelope efficiency
were analyzed by Ultraviolet absorption method. Results: The surface of the microsphere were smooth, the drug loading and envelope efficiency
of lysozyme were 26.83%-41.92% and 15.65%-24.48% respectively. In vitro degradation test revealed that the molecular weight
of chitosan decreased 70.40% in 9 days. SEM demonstrated that the hole diameter of the composite gel with microsphere were uniform
and increased compared with the controlled sample. Conclusions: Such composite scaffolds should be a suitable candidate scaffold model
for loading cartilage related growth factors, which should solve the problem that cartilage tissue engineering is in lack of seeded cells. |
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