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目的：研究P53 Codon 72 多态性、P53 的表达与宫颈癌放疗敏感性的相关性。方法：I b2 期宫颈癌患者274 例，术前192Ir 腔内后装4 次，A 点放疗剂量2400cGy，一周2 次，共2 周。治疗后14d 进行广泛性子宫切除。据术后病理放疗反应HE 染色结果分为放疗敏感与放射抗拒两组。免疫组化SP 法检测P53 蛋白在治疗前宫颈癌组织中的表达，分析放疗敏感与放射抗拒两组P53 蛋白表达差异有无显著性意义；采用PCR 后测序的方法检测治疗前P53 第72 密码子的基因型频率多态性（P53 Codon 72），分析放疗敏感与放射抗拒两组中各P53Codon72 基因型的差异有无显著性意义。结果：放疗抗拒组与放疗敏感组相比，P53 高表达的比例显著高于P53 低表达的比例（P=0.00081）。P53 Codon 72 多态性分析，Pro/Pro 与Arg/Arg、Pro/Pro 与Arg/Pro 在放疗敏感组与放疗抗拒组的分布差异显著（P 值分别为P=0.009 和P=0.032）；Arg/Arg 与Arg/Pro，在放疗敏感组与放疗抗拒组的分布无显著差异（P=0.503）。结论：P53 Codon 72 多态性和P53 蛋白与宫颈癌放疗敏感性有相关性，可以作为早期宫颈癌放疗敏感性的预测指标。

英文摘要:

Objective: To investigate the association of radiotherapy sensitivity to P53 Codon 72 polymorphisms and P53 expression of the cervical cancer. Methods: The Ib2 period cervical cancer 274 samples, preoperative after 192Ir lumen loading four times, radiation dose points A 2400cGy, twice a week for 2 weeks. After 14d of treatment the extensive uterine was dissectioned. These specimens were divided into two groups (radiation resist and radiation sensitive) ,according to the HE dyeing results of the postoperative pathologic radiotherapy reaction specimen. IHC method of SP to detect the P53 protein expression before the treatment of cervical cancer tissue of expression, analysis with radiation resist radiation sensitive P53 protein expression differences between two groups have significant meaning; By PCR and sequencing method to detect P53 codon72 genotype frequency polymorphism (P53 Codon 72) of the samples before treatment, analysis the difference of P53Codon72 genotypes between radiation resist and radiation sensitive group. Results: Compared radiation resist group to radiation sensitive group, the proportion of P53 highly expressed significantly higher than the proportion of P53 lower expression (P = 0.00081). P53 Codon 72 polymorphism analysis，the distribution of Pro/Pro and Arg/Arg、 Pro/Pro and Arg/Pro、Arg/Arg and Arg/Pro in radiotherapy sensitive group and radiation resist group were significant differences (P = 0.946 and P = 0.697 respectively); the distribution of Arg/Arg and Arg/Pro in radiotherapy sensitive group and radiation resist group were no significant difference （P=0.503）. Conclusion: P53 Codon 72 polymorphisms and P53 protein have relativity to cervical cancer radiotherapy sensitivity and can be used as early cervical cancer radiotherapy sensitivity of prediction indexes.

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