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目的：观察生半夏、南星中药水提物对缺氧环境中人胃癌细胞株BGC823 细胞HIF-1α 蛋白表达和侵袭力的影响。方法：运用CoCl2（氯化钴）诱导BCG823 细胞缺氧，使得细胞中HIF-1α 蛋白表达升高. 实验组加入生半夏、南星水提物对细胞进行预处理，然后在进行缺氧诱导。通过甲基噻唑基四唑法（MTT）检测细胞活性，使用Transwell 检测细胞侵袭能力变化，RT-PCR、Western blotting 分别检测HIF-1αmRNA 及蛋白含量及变化。结果：生半夏、南星水提物能抑制人胃癌BGC823 细胞的增殖；生半夏、南星水提物均能抑制缺氧诱导胃癌细胞的侵袭力，并且能降低HIF-1αmRNA 及蛋白表达。结论：生半夏、南星水提物可抑制人胃癌 BGC823 细胞的增殖，抑制人胃癌BGC823 细胞侵袭力，可能通过降低HIF-1α 蛋白表达有关。

英文摘要:

Objective: To investigate the effects of the enthanol and nanxing extract from Rhizome Pinelliae Preparata on hypoxiainducible -1 alpha protein expression and aggressive influence in human gastric cancer cells BGC823. Methods: CoCl2 (chlorinated cobalt) was used to induce BCG823 cytopathic hypoxia approach, which leads the increasing the expression of hypoxia-inducible -1 alpha. The cell viability was detected by MTT chromatometry and cell growth curve was generated; Transwell small room was used to detect cells attack ability, RT-PCR, Western blotting was use to detect hypoxia-inducible-1 alpha mRNA expression and protein level. Results: The extraction of enthanol and nanxing can inhibit the proliferation of cells BGC823 human cancer;.The enthanol and nanxing extract can inhibit the aggressive ability of cells BGC823 human cancer cells, the cause of which may be the decreasing of the expression of HIF-1 alpha mRNA and protein. Conclusion：Enthanol extract from Rhizome Pinelliae Preparata can inhibit the BGC823 cells proliferation and Restrain BGC823 human cancer cells aggressie influence by lowering hypoxia-inducible - 1 alpha mRNAand the protein expression.

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