| 纳冬梅1 孙强2 曹坪1 张宏1 徐波1.大鼠脑缺血再灌注后血清中血管内皮生长因子与神经元凋亡的研究[J].现代生物医学进展英文版,2011,11(10):1847-1850. |
| 大鼠脑缺血再灌注后血清中血管内皮生长因子与神经元凋亡的研究 |
| Relation Between Vascular Endothelial Growth Factor in Sera and NeuronalApoptosis after Cerebral IschemicReperfusion in Rats |
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| DOI： |
| 中文关键词: 血管内皮生长因子 凋亡 脑缺血 大鼠 |
| 英文关键词: R245 vascular endothelial growth factor apoptosis cerebral ischemia rat |
| 基金项目: |
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| 中文摘要: |
| 目的：通过检测SD 大鼠脑缺血再灌注模型血清中血管内皮生长因子(VEGF) 与神经元凋亡动态表达变化的关系，以探讨
两者之间的相关性。方法：将40 只大鼠随机分为8 组：对照组、假手术组和脑缺血30 min 再灌注12 h 组、1d 组、3 d 组、5d 组、7d
组、及14 d 组，每组5 只。采用ELISA 双抗夹心法检测大鼠血清中血管内皮生长因子、原位细胞凋亡TUNEL 法检测脑组织中的
凋亡神经细胞数。结果：再灌注12h、1d、3 d、5d、7d 及14 d 大鼠血清VEGF 表达和凋亡神经元百分比的变化均为负相关性(均为
P<0.05 )。结论：在脑缺血再灌注大鼠模型中，缺血诱导使VEGF 的表达发生变化，VEGF 通过直接或间接的途径抑制神经元凋
亡。 |
| 英文摘要: |
| Objective: To investigate the interrelation ship between thedynamic changes of of VEGF and neuronal apoptosis after
Cerebral Ischemic Reperfusion in SD Rats. Methods: A total of forty SD rats were randomly divided into eight different groups （n = 5):
controls group, sham-operation group，cerebral ischemia for 30 min and reperfusion for 12 hour( I/R12h group)，1day(I/R1d group)，3 day
(I/R3d group)，5 day( I/R5d group)，7 day( I/R7d group), and 14day( I/R14d group). By using ELISA method the VEGF were measured，
the apoptosis of neurons was examined by TUNEL. Results: The expression of VEGF has a negative correlation （P<0.05) with neurons
apoptosis reperfusion for 12hour，1day，3 day，5day，7day and 14day after Cerebral Ischemic Reperfusion. Conclusions: VEGF mediated
by cerebral ischemia inhibits the apoptosis of neurons in rats after cerebral ischemic reperfusion. |
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