| 张凯马泽刚王俊谢安木.杨梅黄酮减弱MPP+ 诱导的MES23.5 细胞的细胞毒性[J].现代生物医学进展英文版,2011,11(10):1830-1833. |
| 杨梅黄酮减弱MPP+ 诱导的MES23.5 细胞的细胞毒性 |
| Myricetin Attenuates MPP+-induced Cytotoxicity in MES23.5 Cells |
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| DOI： |
| 中文关键词: 杨梅黄酮 MPP+ 凋亡 MES23.5 cells |
| 英文关键词: Myricetin MPP+ Apoptosis MES23.5 cells |
| 基金项目:国家自然科学基金项目（No. 30800353），青岛市科技局项目（09-1-1-29-nsh） |
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| 中文摘要: |
| 目的：探讨杨梅黄酮对MPP+ 处理的MES23.5 细胞的保护作用。方法: 实验分为空白对照组，MPP+ 处理组，MPP+ 和杨梅黄
酮共处理组，分别处理MES23.5 细胞24h 后，应用Hoechst 33258 染色观察各组细胞细胞核形态的改变，应用RT-PCR 技术观察
Bcl-2 和Bax mRNA 表达的改变。结果: 杨梅黄酮能明显抑制MPP+ 引起的MES23.5 细胞核固缩，核碎裂等形态学的改变，杨梅
黄酮还可以对抗MPP+ 处理造成的MES23.5 细胞的Bcl-2/Bax 比率的降低。结论: 杨梅黄酮对MPP+ 处理的MES23.5 细胞具有保
护作用。 |
| 英文摘要: |
| Objective: To investigate the neuroprotective effect of myricetin on 1-methyl-4-phenylpyridinium (MPP+)-treated
MES23.5 cells. Methods: MES23.5 cells were treated with vehicle, MPP+, MPP+ and myricetin for 24h respectively. By Hoechst staining
and RT-PCR, the nuclear morphological changes and the Bcl-2 and Bax mRNA levels were investigated. Results: The results showed
that myricetin treatment significantly attenuate MPP+-induced cell apoptosis. MPP+ treatment induced decrease of Bcl-2/Bax mRNA ratio
was also inhibited by myricetin. Conclusion: Myricetin may protect against MPP+-induced cytotoxicity in MES23.5 cells. |
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