胡琳洁1 郭晓雅1 侯美娜1 邵成2 史皆然1.评价空肠弯曲菌外膜蛋白PEB1 介导的重组BCG 与上皮细胞
的结合能力[J].现代生物医学进展英文版,2011,11(10):1810-1812. |
评价空肠弯曲菌外膜蛋白PEB1 介导的重组BCG 与上皮细胞
的结合能力 |
Evaluation of Combination Ability Between Recombinant BCG Mediated byMembrane Protein PEB1 of C.Jejuni and Epithelium Cells |
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DOI: |
中文关键词: 重组BCG 细胞黏附 HeLa 细胞 HIEC |
英文关键词: Recombinant BCG Adherence HeLa cell HIEC |
基金项目:国家自然科学基金资助(30871114) |
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中文摘要: |
目的:评价PEB1 介导的屋尘螨抗原(Der p2)重组BCG 疫苗(PEB1-Der p2- rBCG)与人上皮细胞的结合能力。方法:采用体
外细胞培养的方法,分别将普通BCG、胞壁型Der p2-rBCG 和胞壁型融合蛋白PEB1-Der p2- rBCG 与HeLa 细胞及人类肠粘膜上
皮细胞(HIEC)进行共孵育,利用HE 和抗酸染色法对各组细胞与疫苗的黏附结果进行染色,光学显微镜下计数各组的黏附率,并
进行比较;对以上各组分别加入PEB1 蛋白,进行黏附阻断,观察对结合能力的影响。结果:孵育24 小时后,无论HeLa 细胞还是
HIEC PEB1-Der p2- rBCG 组较普通BCG 组和Der p2-rBCG 组的黏附率明显提高,差异有显著性(P<0.05);PEB1 蛋白的加入对
PEB1-Der p2- rBCG 的黏附功能有明显抑制作用(P<0.05);但是,Der p2 -rBCG 组与普通BCG 组比较没有明显差异(P>0.05),
PEB1 蛋白的加入对二者的黏附亦无影响(P>0.05)。结论:PEB1 具有介导增强PEB1-Der p2- rBCG 与上皮细胞黏附的能力。 |
英文摘要: |
Objective: To investigate the combination ability of the PEB1-Der p2- rBCG and epithelium cells. Methods: The
method of cell culture in vitro was used to culture the bacterial strains of common BCG,membrane form Der p2-rBCG and membrane
form PEB1-Der p2- rBCG with HeLa and human intestinal epithelial cells (HIEC),HE staining and acid-fast staining were used and the
adhesion of cells was detected at light microscopy; purified PEB1was added into each group to adhesion blocking,and the influence of
combining ability was observed. Results: After incubated for 24 h,no matter HeLa cells or HIEC,adhesion rate of PEB1-Derp2- rBCG
was obviously improved than common BCG and Der p2-rBCG, and the differences were statistically significant (P < 0.05); The
differences between PEB1-Der p2- rBCG performed in the absence and presence of PEB1 protein were significant (P < 0.05); However,
the Der p2-rBCG bacteria group was not significantly different from common BCG group (P > 0.05) and no adhesion blocking effects in
the two group which added PEB1 protein (P > 0.05). Conclusion: The PEB1 increased the adhesion ability which mediated PEB1- Der p2
- rBCG and epithelium cells. |
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