| 石炜曾斌△ 张杰谈高熊丹.NF-kB 拮抗剂PDTC 对HepG2 细胞的抑制及对caspase-3 表达的影响[J].现代生物医学进展英文版,2011,11(9):1651-1654. |
| NF-kB 拮抗剂PDTC 对HepG2 细胞的抑制及对caspase-3 表达的影响 |
| Effect of NF-kB Antagonist PDTC in Inhibition of Human HepaticCarcinoma Cell HepG2 |
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| DOI： |
| 中文关键词: PDTC 人肝癌HepG2 细胞 caspase-3 |
| 英文关键词: PDTC human hepatic carcinoma cell HepG2 caspase-3 |
| 基金项目: |
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| 中文摘要: |
| 目的：研究NF-kB 拮抗剂PDTC 诱导人肝癌HepG2 细胞凋亡及其对凋亡相关基因caspase-3 表达的影响，初步探讨其诱导
凋亡的可能机制。方法：以不同浓度的PDTC 处理人肝癌HepG2 细胞，利用MTT 法检测对人肝癌HepG2 细胞凋亡的影响；利用
RT-PCR 和Western-blot 检测caspase-3 mRNA 和蛋白的表达。结果：不同浓度PDTC 作用人肝癌HepG2 细胞不同时间后，能够显
著抑制HepG2 细胞的生长增殖，存在剂量和时间依赖性（P<0.05）; PDTC 能够上调HepG2 细胞中caspase-3 mRNA 和蛋白的表
达。结论：NF-kB 拮抗剂PDTC 对人肝癌HepG2 细胞产生显著抑制，并能上调HepG2 细胞中caspase-3 mRNA 和蛋白的表达。 |
| 英文摘要: |
| Objective: To investigate the role of apoptosis by PDTC,the selective inhibit of NF-kB on human hepatic carcinoma
cell (HepG2). and the effect of expression of csaspase-3,principium disguisition the likelymechalism which induce HepG2 cells apoptosis.
Methods: After treating human hepatic carcinoma cell (HepG2) by PDTC, MTT assay was used to observe the growth inhibition of cell-
HepG2. The mRNA and protein expression of caspase-3 were detected by RT-PCR and Western-blot assay, respectively. Results: When
PDTC was given, growth inhibition and elevated apoptosis of HepG2 cells were detected,which was showed in a dose- and time- - dependent
manner, PDTC up-regulated the lever of mRNA and protein expression of caspase-3. Conclusion: PDTC can induce apoptosis of
human hepatic carcinoma cell HepG2 cells,which may be performed by up-regulated of caspase-3 expression. |
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