马晓蕾潘峰胡丹.视神经损伤后视网膜Müller 细胞未折叠蛋白反应
相关因子IRE-1 与GLAST 的表达[J].现代生物医学进展英文版,2011,11(8):1447-1450. |
视神经损伤后视网膜Müller 细胞未折叠蛋白反应
相关因子IRE-1 与GLAST 的表达 |
Expression of Unfolded Protein Reaction Related Factor IRE-1 and GLASTin Retinal Müller Cell after Optic Nerve Injury |
|
DOI: |
中文关键词: 未折叠蛋白反应 Müller 细胞 GLAST 视网膜 大鼠 |
英文关键词: UPR Müller cells GLAST Retina Rat |
基金项目:国家自然科学基金(30772368) |
|
Hits: 696 |
Download times: 879 |
中文摘要: |
目的:研究视神经损伤后视网膜Müller 细胞中是否有未折叠蛋白反应(UPR)及其与L 一谷氨酸/L 一天门冬氨酸转运体
(GLAST)的关系。方法:视神经钳夹伤模型建立成功后,运用HE 染色观察视网膜神经节细胞数目改变,免疫化学染色,免疫荧光
双标记,western-blot 观察UPR 相关因子需肌醇酶1 (IRE-1) 与GLAST 的表达及相关性。结果:视神经钳夹伤后IRE-1 与
GLAST 在视网膜Muller 细胞上共表达,,术后第一天前呈上升趋势,在第一天达到顶峰,后呈下降趋势,第七天下降明显。结论:
视神经损伤后,IRE-1 与GLAST 的趋势变化有一定相关性,提示未折叠蛋白反应可能是调控GLAST 变化的原因之一。 |
英文摘要: |
Objective: To investigate the unfolded protein response (UPR)in retinal Müller cells after optic nerve injury and the
relationship between UPR and glutamate/aspartate transporter (GLAST). Methods: The change of the amount of retina gangliocyte was
detected by HE dying and the expression and relevance of UPR correlation factor-inositol requiring enzyme 1 (IRE-1)and GLAST were
detected by immunohistochemical staining, immunofluorescent double-tagging, western-blot after the modeling of trapped wound in
optic nerve nipper. Results:IRE-1 and GLAST co-express in retinal Müller cells, ascend in the common trend and reach the summit one
day after surgery, then drop sharply in the seventh day.Conclusions: There was relationship between the IRE-1 and GLAST in trend
changes after optic nerve injured which suggests UPR may be one cause of the regulation of GLAST changes. |
View Full Text
View/Add Comment Download reader |
Close |