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目的：探讨双酚A（BPA）对成年大鼠抗氧化能力的影响。方法：将健康成年雄性SD 大鼠84 只随机分为5 个双酚A 染毒剂量组（200、50、5、0.5、0.0005 mg/kg）和1 个对照组，连续灌胃染毒8 周，并监测体重变化，染毒结束后测定血浆超氧化物歧化酶（SOD）和谷胱甘肽- 过氧化物酶（GSH-Px）含量；此外，提取肝脏组织RNA，用荧光实时定量PCR 测定肝脏硫氧还原蛋白过氧化物酶2（prdx2）mRNA 的表达水平。结果：随着染毒时间的延长, 50 mg/kg 及200 mg/kg 剂量组的动物体重增长速度逐渐减慢，自4 周开始，与对照组相比有显著差异（P<0.05）；染毒结束时，其体重分别为对照组的90.52 %和91.61 %（p <0.05），而其它低剂量组间则无明显差异；大鼠血浆GSH-Px 含量总体上呈下降趋势，且5 mg/kg 以上剂量组与对照组相比差别均有统计学意义(p<0.01)；染毒组血浆SOD 水平虽也低于对照组，但无统计显著性；此外，大鼠肝脏组织prdx2 mRNA 水平随双酚A 剂量增高呈剂量依赖性降低，与对照组相比，其在最低剂量组（0.0005 mg/kg）即有显著差异（p<0.01）。结论：双酚A 可造成大鼠抗氧化酶系统失衡，导致氧化损伤效应，prdx2 可能是反映其氧化损伤敏感的生物学标志。

英文摘要:

Objective: To investigate the effects of bisphenol A (BPA) on antioxidant capacity of adult Sprague-Dawley rats. Methods: A total of 84 healthy adult male SD rats were divided into 5 different concentration of BPA groups (200, 50, 5, 0.5, 0.0005 mg/kg/d) and a control group. The weight of the rats was detected, and the levels of SOD and GSH-Px in the plasma was detected. The mRNA of prdx2 was determined by real-time PCR. Results: The increasing of weight in 50 and 200 mg/kg/d BPA groups got slow day after day from the 4th week; At the end of the experiment, the weight of the two groups reached 90.52 % and 91.61 % of that of control (p<0.05). Plasma GSH-Px had declined in all groups except 0.0005 mg/kg/d compared with that of the control. All BPA groups of plasma SOD were lower than that of control group; The levels of prdx2 mRNA in the rat liver was significant dose-responsed, with BPA increasing ,the mRNA decreased. Conclusions: BPA caused unbalance of antioxidant enzyme system of adult male SD rats, and led to oxidative damage. Prdx2 may be the sensitive biomarker of oxidative damage.

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