| 景蓓蓓杨晓红张胜海答敏张萍朱依娜.基于钙黄绿素的荧光分光光度法测定谷胱甘肽[J].现代生物医学进展英文版,2011,11(7):1359-1362. |
| 基于钙黄绿素的荧光分光光度法测定谷胱甘肽 |
| Determination of Glutathione by Fluorescence SpectroscopyMethod Basedon Calcein-Copper(Ⅱ) System |
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| DOI： |
| 中文关键词: 谷胱甘肽 荧光分光光度法 钙黄绿素 |
| 英文关键词: Glutathione Fluorescence Calcein |
| 基金项目: |
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| 中文摘要: |
| 目的：基于钙黄绿素的荧光分光光度法建立一种测定谷胱甘肽的新方法。方法：在pH=8.0 介质中, 铜(Ⅱ)与钙黄绿素配位引
起荧光猝灭,由于谷胱甘肽与铜(Ⅱ)的亲和力很强，可从钙黄绿素- 铜离子的络合物中夺取铜离子而使钙黄绿素游离出来使荧光
强度得以恢复，荧光恢复的程度与加入谷胱甘肽的浓度在一定浓度范围内成线性。结果：据此建立了一种测定谷胱甘肽的新方法,
该方法的线性范围为2.0×10-6~1.4×10-5mol·L-1，F = 8.1964C+ 196.43，检测限为1.0×10-6mol/L。结论：用此法测定谷胱甘肽简便
快速，灵敏度高。 |
| 英文摘要: |
| Objective: To establish a new method of measuring reduced glutathione by calcein copper (Ⅱ)system. Methods: In
pH=8.0 buffer solution, Copper (Ⅱ) can coordinate with calcein, and cause the fluorescence intensity of calcein solution decreased.
Because of the affinity between Copper (Ⅱ) and reduced glutathione is very strong, so it can seize copper ions from calcein-Copper (Ⅱ)
complex and make calcein free, thus make the fluorescence recovered, and the recovery degree of its fluorescence was linear related to
the concentration of reduced glutathione added into the solution. Results: Hereby established a new method of measuring reduced
glutathione in the range of with 2.0×10-6~1.4×10-5 mol·L-1，F = 8.1964C+ 196.43，a detect limit of 1.0×10-6mol/L. Conclusion: This
method of measuring reduced glutathione is simple, quick and sensitive. |
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