| 杨晓红景蓓蓓张胜海答敏张萍朱依娜.基于钙黄绿素- 铜(Ⅱ)荧光体系测定乙酰半胱氨酸[J].现代生物医学进展英文版,2011,11(7):1355-1358. |
| 基于钙黄绿素- 铜(Ⅱ)荧光体系测定乙酰半胱氨酸 |
| Fluorimetric Determination of Acetylcysteine Based onCalcein-Copper(Ⅱ) System |
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| DOI： |
| 中文关键词: 钙黄绿素 荧光 乙酰半胱氨酸 |
| 英文关键词: Calcein Fluorescence Determination Acetylcysteine |
| 基金项目: |
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| 中文摘要: |
| Fluorimetric Determination of Acetylcysteine Based on
Calcein-Copper(Ⅱ) System |
| 英文摘要: |
| Objective: Fluorimetric Determination of Acetylcysteine in Calcein-Copper ( Ⅱ ) System. Methods: In pH=8.0 of
KH2PO4-Na2HPO4·12H2O buffer solution, determinating the fluorescence intensity of acetylcysteine solutions with 492nm as excitation
wavelength and 520nm as emission wavelength. Rusults: In pH=8.0 of KH2PO4-Na2HPO4·12H2O buffer solution, copper (Ⅱ) can
coordinate with calcein, and cause the fluorescence intensity of calcein solution decreased. Because the appetency between copper (Ⅱ)
and acetylcysteine was very strong, it can seize copper ions from calcein-copper (Ⅱ) complex and make calcein free, thus make the
fluores- cence recovered, and the recovery degree of its fluorescence was linear related to the concentration of acetylcysteine added to the
solution. Conclusions: Hereby we establish a new method of measuring acetylcysteine in the range of 6.0 10-6~1.4 10-5 mol/L. The
detection limit was 4.0×10-6 mol/L. The relative standard deviation was 0.91 % for the 11 replicate determinations of 1.0×10-5 mol/L
acetylcysteine solution. This method was applied to the determination of acetylcysteine in pharmaceutical preparations. |
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