| 孙红英1 方菲1 赵露晶2 邢国杰1 沈鹤柏1.神经元特异性烯醇酶和癌胚抗原胶体金免疫层析联合检测[J].现代生物医学进展英文版,2011,11(7):1261-1265. |
| 神经元特异性烯醇酶和癌胚抗原胶体金免疫层析联合检测 |
| Joint Detection of Neuron-specific Enolase and Carcinoembryonic Antigenwith Gold Immunochromatography Assay（GICA） |
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| DOI： |
| 中文关键词: NSE CEA 联合检测 胶体金 免疫层析 |
| 英文关键词: NSE CEA Joint detection Colloidal gold Immunochromatography |
| 基金项目:上海市科委项目( 0752nm028 )，（08JC140600），（0952nm028） |
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| 中文摘要: |
| 目的：应用双抗夹心胶体金免疫层析方法，实现对神经元特异性烯醇化酶（NSE）和癌胚抗原（CEA）两种肺癌肿瘤标志物的
快速联合检测。方法：采用柠檬酸三钠还原法制备20nm 胶体金颗粒，并分别对鼠抗NSE、CEA 单克隆抗体进行标记，分别与之相
配对的另一种单克隆抗体被喷在硝酸纤维素膜（NC 膜）上，制成免疫层析检测试条。溶液中的抗原NSE、CEA 与金标记抗体结合
后沿着硝酸纤维素膜移动，与膜上固定的抗体结合形成肉眼可见的红色线条。结果：该试纸条只与NSE、CEA 有特异性反应，与
CA125、CYFRA21-1、TPA 等肺癌标志物无交叉反应。标准样品中两种抗原的检测灵敏度分别可达到5ng/mL 和3ng/mL。结论：胶
体金免疫层析技术检测NSE、CEA 特异性强、灵敏度高、简便快速，不需特殊仪器设备，有广泛应用价值。 |
| 英文摘要: |
| Objective: To realize the rapid joint detection for Neuron-specific enolase and carcinoembryonic antigen by double
antibodies sandwich gold immunochromatography assay. Methods: Colloidal gold with diameter 20nm was prepared through reducing
chloroauric acid with trisodium citrate. Gold nanoparticles were coupled with monoclonal anti-NSE/CEA. Another corresponding
monoclonal antibody to the two antigens was fixed on the NC membrane to prepare GICA test strips. The gold-anti-NSE/CEA conjugate
reacts with NSE/CEA in solution and migrates on the nitrocellulose membrane on which anti-NSE/CEA was immobilized, and producd
the visual red lines. Results: This kind of test strips could react with NSE and CEA specificly, and there were no cross-reactions with
other lung cancer markers, such as CA125, TPA, CYFRA21-1. The sensitivity of NSE was 5ng/mL and CEA was 3ng/mL by detecting
the standard samples. Conclusion: GICA is a sensitive, specific,simple and rapid assay for detecting NSE and CEA. It need not any
special devices and has significant practical value. |
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