| 栾春业1 王强2 张晶1 徐艳2 肖杭2△ 张劲松1.六氯苯对PC-12 细胞凋亡的影响[J].现代生物医学进展英文版,2011,11(7):1216-1219. |
| 六氯苯对PC-12 细胞凋亡的影响 |
| Effects of Hexachlorobenzene on the Apoptosis of PC-12 Cells |
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| DOI： |
| 中文关键词: 环境内分泌干扰物 六氯苯 PC-12 细胞 凋亡 p-PLCγ p-ERK1/2 |
| 英文关键词: EDCs Hexachlorobenzene PC-12 cells Apoptosis p-PLC p-ERK1/2 |
| 基金项目:国家自然科学基金项目（81072329） |
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| 中文摘要: |
| 目的：探讨环境内分泌干扰物六氯苯对PC-12 细胞凋亡的影响及其可能机制。方法：采用体外细胞培养方法，5 % CO2 37 ℃
恒温条件下，将PC-12 细胞培养于含10％灭活胎牛血清的高糖DMEM 培养基中。0、1、10、20、100、200 μmol.L-1 六氯苯处理组，
每组设3 组复孔，培养24 h 后应用Cell Counting Kit-8 试剂盒进行六氯苯对PC-12 细胞增殖和毒性分析；流式细胞术FITC-AnnexinV/
PI 双染检测细胞凋亡率；Hoechst33258 染色及倒置荧光显微镜拍摄细胞平片，观察细胞形态学改变；免疫印记法（Western
blot） 检测相关蛋白PLCγ 及ERK 蛋白磷酸化表达变化。结果：随着六氯苯浓度增加，细胞凋亡率升高，呈剂量依赖关系；
Hoechst33258 染色可见细胞核膨大、染色质边集浓染等凋亡特征；与对照组相比p-PLCγ 及p-ERK1/2 表达均降低，呈剂量依赖
效用。结论：六氯苯能够诱导PC-12 细胞凋亡，p-PLCγ及p-ERK1/2 信号通路可能在此过程中发挥作用。 |
| 英文摘要: |
| Objective: To investigate the effects of Hexachlorobenzene on the apoptosis of PC-12 cells. Methods: The PC-12 cells
were cultured in high glucose Dulbecco's modified Eagle's medium (DMEM) and supplemented with 10 % fetal bovine serum, 100 μg.
ml-1 penicillin, and 100 μg.ml-1 streptomycin at 37℃ in a 5% CO2 atmosphere. Treated with Hexachlorobenzene (0,1,10,20,100, 200
μmol.L-1) for 24 hours. Cell Counting Kit-8 was used to detected the proliferation and to do toxicity analysis. The morphological
changes of PC-12 cells were observed by microscope, and cell apoptosis rates were detected by flow cytometry. The expression of
p-PLCγ and p-ERK1/2 were determined by Western blot. Results: After treated with Hexachlorobenzene, PC-12 cells had morphological
changes and higher apoptosis rates. The changes was evident with the increasing of the dose of Hexachlorobenzene. The 50% inhibiting
concentration was 72 μmol.L-1. The apoptosis rate of 200 μmol.L-1 was 87.12 ± 3.21%. Conclusions: Hexachlorobenzene could induce
apoptosis of PC-12 cells, and the expression of p-PLCγ and p-ERK1/2 proteins may play a role in the process of PC-12 cells apoptosis
induced by Hexachlorobenzene. |
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