| 李庆1 陈城2 谢利平2 刘晓燕2 施辉1.螺内酯对单肾切除糖尿病大鼠肾脏保护作用研究[J].现代生物医学进展英文版,2011,11(6):1097-1101. |
| 螺内酯对单肾切除糖尿病大鼠肾脏保护作用研究 |
| Protection of Spironolactone on the Kidney in Diabetic Rats UndergoingMono-nephrectomy |
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| DOI： |
| 中文关键词: 螺内酯 肝细胞生长因子（HGF） 糖尿病肾病（DN） |
| 英文关键词: Spironolactone Hepatocyte growth factor (HGF) Diabetic nephropathy(DN) |
| 基金项目:南通市社会发展科技计划：螺内酯对单肾切除糖尿病大鼠的保护机制（s2008054） |
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| 中文摘要: |
| 目的：研究螺内酯对单肾切除糖尿病SD 大鼠的肾脏保护作用及机制。方法：48 只雄性SD 大鼠随机分为单肾切除对照组C
组、单肾切除糖尿病模型组D 组、单肾切除糖尿病螺内酯干预组S 组（50mg/kg/day 灌胃）。给药6 周后观察各组大鼠生化指标改
变，肾组织形态学改变，RT-PCR 及Western Blot 检测肾组织中HGF 水平的变化。结果：给药6 周后，D 组与C 组相比，血糖、24h
尿蛋白定量、尿素氮、血肌酐和肾重/ 体重显著增高（P<0.05），螺内酯治疗后可降低糖尿病大鼠的24h 尿蛋白定量和肾重/ 体重，
但对其它指标无明显影响；形态学显示，D 组与C 组相比肾小球系膜区细胞外基质增多及系膜细胞增生，肾小球基底膜增厚，足
细胞足突融合、消失；螺内酯治疗后上述病理改变均有不同程度的减轻；RT-PCT、Western Blot 结果显示D 组和S 组HGF 表达均
增加（P<0.05），而D 组HGF 表达明显低于螺内酯治疗组。结论：螺内酯可能通过上调HGF 的水平从而起到保护糖尿病大鼠肾脏
作用。 |
| 英文摘要: |
| Objective: To investigate the effect of spironolactone on the mono-nephrectomy diabetic SD rats and to explore the
possible mechanism. Methods: 48 male SD rats were randomly divided into three groups: mono-nephrectomy control rats (group C),
mono-nephrectomy diabetic rats (group D), and mono-nephrectomy diabetic rats treated with spironolactone (group S, 50mg/kg/day,
intragastric administration). 6 weeks after treatment, biochemical indicators and renal tissue morphology were detected. The mRNA and
protein expression levels of HGF were detected by RT-PCR and Western Blot respectively. Results: Blood sugar, 24 hours urinary
protein, serum creatinine (SCr), urea nitrogen (BUN), and ratio of renal weight/ body weight increased in mono-nephrectomy diabetic rats
(group D) compared with those in the mono-nephrectomy control rats (group C) (P<0.05). Spironolactone could decrease the 24 hours
urinary protein and ratio of renal weight/ body weight, but had no obvious effect on the other indicators in diabetic rats. Compared with
group C, there was the expansion of extracellular matrix with proliferated intercapillary cells in the expanded mesangial area, the
thickening of basement membrane (GBM) in group D.Foot processes even fused or disappeared in the rats of group D. Meanwhile, the
renal pathologic changes as above were improved in the rats of group S..The mRNA and expression of HGF were up-regulated in the rats
of group D and S, especially in the group S (P<0.05). Conclusion: Spironolactone ameliorates the glomerular injury in diabetic rats
presumably via up-regulation of HGF expression level. |
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